Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients.

Autor:	Diniz TG; Post-Graduate Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil., Silva AS; Department of Physical Education, Federal University of Paraiba (UFPB), Joao Pessoa, Brazil., Dos Santos Nunes MK; Post-Graduation Program in Development and Technological Innovation of Medicines (DITM), Federal University of Paraiba, Joao Pessoa, Brazil., Ribeiro MD; Post-Graduation Program in Physical Education, Federal University of Paraiba, Joao Pessoa, Brazil., Filho JM; Department of Internal Medicine, Federal University of Paraiba, Joao Pessoa, Brazil., do Nascimento RAF; Uninassau Faculty, Mauricio de Nassau University, João Pessoa, Brazil., Gomes CNAP; Nephrology Clinic, Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Brazil., Evangelista IWQ; Ophthalmology Reference Center, Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Brazil., de Oliveira NFP; Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa, Brazil., Persuhn DC; Department of Molecular Biology and Post-Graduation Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2021 Jan 12; Vol. 11, pp. 618672. Date of Electronic Publication: 2021 Jan 12 (Print Publication: 2020).
DOI:	10.3389/fphys.2020.618672
Abstrakt:	Introduction: MTHFR methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown. Objective: The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of the MTHFR gene in patients with type 2 diabetes mellitus (T2DM). Methods: A total of 111 patients, 43 men and 68 women diagnosed with DM (7.0 ± 2.3 years), answered the International Physical Activity Questionnaire (IPAQ) and underwent blood collection for biochemical analysis, DNA extraction, and MTHFR gene methylation profile determination. Result: The comparison of the methylation pattern showed that the partially methylated profile predominates in the insufficiently active group (85%), which does not occur in the sufficiently active group (54%) ( p = 0.012). No differences were found in the nutritional status comparison. Logistic regression including overweight, waist circumference, gender, age, time of DM, hypertension, dyslipidemia, smoking, alcoholism, and family DM revealed that the association of the level of physical activity with methylation profile proved to be independent of these confounding variables. Considering the partially methylated profile as a result, being physically inactive favors the partially methylated MTHFR pattern in patients with DM. Conclusion: We concluded that insufficient physical activity is associated with partially methylated pattern of MTHFR promoter. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Diniz, Silva, dos Santos Nunes, Ribeiro, Filho, do Nascimento, Gomes, Evangelista, de Oliveira and Persuhn.)
Databáze:	MEDLINE
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