Anti-Fatigue Activity of a Mixture of Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. Fruit Extract.

Autor: Oh J; Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd., Gyeonggi 13486, Korea., Han Y; Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd., Gyeonggi 13486, Korea., Kim J; Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd., Gyeonggi 13486, Korea., Park C; Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd., Gyeonggi 13486, Korea., Oh D; Jeollanamdo Institute of Natural Resources Research, Jeonnam 59338, Korea., Yun H; Jeollanamdo Institute of Natural Resources Research, Jeonnam 59338, Korea., Lee G; Jeollanamdo Institute of Natural Resources Research, Jeonnam 59338, Korea., Kim J; Jeollanamdo Institute of Natural Resources Research, Jeonnam 59338, Korea., Choi C; Jeollanamdo Institute of Natural Resources Research, Jeonnam 59338, Korea., Lee Y; Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd., Gyeonggi 13486, Korea.
Jazyk: angličtina
Zdroj: Preventive nutrition and food science [Prev Nutr Food Sci] 2020 Dec 31; Vol. 25 (4), pp. 380-388.
DOI: 10.3746/pnf.2020.25.4.380
Abstrakt: Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. are commonly used in traditional herbal medicine and food and both exhibit antioxidant and anti-inflammatory effects. Herein, hot-water extracts of Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. fruits (1:1 mixture) were used to produce a complex extract NET-1601. The anti-fatigue activity of NET-1601 was evaluated in an in vitro oxidative stress model induced by treating C2C12 myotubes with H 2 O 2 . An exhaustive swimming test (EST) in vivo model was established using ICR mice. NET-1601-treated C2C12 myotubes (50, 100, and 200 mg/mL) with H 2 O 2 -induced oxidative stress displayed significantly increased cell viability and ATP content, but significantly decreased levels of reactive oxygen species. All NET-1601-treated EST models demonstrated significantly higher maximum swimming rates than control mice. Furthermore, serum lactate, lactate dehydrogenase activity, non-esterified fatty acid, and intramuscular glycogen levels were higher in NET-1601-treated mice than in control mice. In addition, mRNA levels of regulatory factors involved in muscle mitochondrial fatty acid β-oxidation increased upon NET-1601 treatment. Moreover, catalase, superoxide dismutase, glutathione- S -transferase, and liver glutathione content, and antioxidant activity were higher in NET-1601-treated mice than in control mice. Reduced malondialdehyde levels indicated that NET-1601 treatment inhibited exercise-induced lipid peroxidation. Together, these results suggest that NET-1601 retains antioxidant enzyme activity during oxidative stress, simultaneously enhancing both muscle function via glycogen and fatty acid oxidation, thereby exerting a positive effect on recovery from fatigue.
Competing Interests: AUTHOR DISCLOSURE STATEMENT The authors declare no conflict of interest.
(Copyright © 2020 by The Korean Society of Food Science and Nutrition. All rights Reserved.)
Databáze: MEDLINE
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