Detection of ESBL/AmpC-Producing and Fosfomycin-Resistant Escherichia coli From Different Sources in Poultry Production in Southern Brazil.

Autor: Gazal LES; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil., Medeiros LP; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil., Dibo M; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil., Nishio EK; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil., Koga VL; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil., Gonçalves BC; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil., Grassotti TT; Postgraduate Program in Animal Health, Avian Health Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture Livestock Rural and Development, Eldorado do Sul, Brazil., de Camargo TCL; Postgraduate Program in Animal Health, Avian Health Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture Livestock Rural and Development, Eldorado do Sul, Brazil., Pinheiro JJ; Postgraduate Program in Animal Health, Avian Health Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture Livestock Rural and Development, Eldorado do Sul, Brazil., Vespero EC; Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, Londrina, Brazil., de Brito KCT; Postgraduate Program in Animal Health, Avian Health Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture Livestock Rural and Development, Eldorado do Sul, Brazil., de Brito BG; Postgraduate Program in Animal Health, Avian Health Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture Livestock Rural and Development, Eldorado do Sul, Brazil., Nakazato G; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil., Kobayashi RKT; Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina, Brazil.
Jazyk: angličtina
Zdroj: Frontiers in microbiology [Front Microbiol] 2021 Jan 11; Vol. 11, pp. 604544. Date of Electronic Publication: 2021 Jan 11 (Print Publication: 2020).
DOI: 10.3389/fmicb.2020.604544
Abstrakt: This study discussed the use of antimicrobials in the commercial chicken production system and the possible factors influencing the presence of Extended-spectrum β-lactamase (ESBL)/AmpC producers strains in the broiler production chain. The aim of this study was to perform longitudinal monitoring of ESBL-producing and fosfomycin-resistant Escherichia coli from poultry farms in southern Brazil (Paraná and Rio Grande do Sul states) and determine the possible critical points that may be reservoirs for these strains. Samples of poultry litter, cloacal swabs, poultry feed, water, and beetles ( Alphitobius sp.) were collected during three distinct samplings. Phenotypic and genotypic tests were performed for characterization of antimicrobial resistant strains. A total of 117 strains were isolated and 78 (66%) were positive for ESBL production. The poultry litter presented ESBL positive strains in all three sampled periods, whereas the cloacal swab presented positive strains only from the second period. The poultry litter represents a significant risk factor mainly at the beginning poultry production (odds ratio 6.43, 95% confidence interval 1-41.21, p < 0.05). All beetles presented ESBL positive strains. The predominant gene was bla CTX-M group 2, which occurred in approximately 55% of the ESBL-producing E. coli . The cit gene was found in approximately 13% of the ESBL-producing E. coli as AmpC type determinants. A total of 19 out of 26 fosfomycin-resistant strains showed the fos A3 gene, all of which produced ESBL. The correlation between fos A3 and bla CTX-M group 1 ( bla CTX-M55 ) genes was significant among ESBL-producing E. coli isolated from Paraná (OR 3.66, 95% CI 1.9-9.68) and these genetic determinants can be transmitted by conjugation to broiler chicken microbiota strains. Our data revealed that poultry litter and beetles were critical points during poultry production and the presence of fosfomycin-resistant strains indicate the possibility of risks associated with the use of this antimicrobial during production. Furthermore, the genetic determinants encoding CTX-M and fosA3 enzymes can be transferred to E. coli strains from broiler chicken microbiota, thereby creating a risk to public health.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Gazal, Medeiros, Dibo, Nishio, Koga, Gonçalves, Grassotti, de Camargo, Pinheiro, Vespero, de Brito, de Brito, Nakazato and Kobayashi.)
Databáze: MEDLINE
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