Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium.

Autor:	Gavrilov SN; Winogradsky Institute of Microbiology, FRC Biotechnology Russian Academy of Sciences, Moscow, Russia., Zavarzina DG; Winogradsky Institute of Microbiology, FRC Biotechnology Russian Academy of Sciences, Moscow, Russia., Elizarov IM; Winogradsky Institute of Microbiology, FRC Biotechnology Russian Academy of Sciences, Moscow, Russia., Tikhonova TV; Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Dergousova NI; Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Popov VO; Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Kurchatov Complex NBICS-Technologies, National Research Center 'Kurchatov Institute,' Moscow, Russia., Lloyd JR; Dalton Nuclear Institute, FSE Research Institutes, The University of Manchester, Manchester, United Kingdom., Knight D; Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom., El-Naggar MY; University of Southern California, Los Angeles, CA, United States., Pirbadian S; University of Southern California, Los Angeles, CA, United States., Leung KM; University of Southern California, Los Angeles, CA, United States., Robb FT; School of Medicine, University of Maryland, Baltimore, Baltimore, MD, United States., Zakhartsev MV; Faculty of Biosciences, Norwegian University of Life Sciences, Ås, Norway., Bretschger O; J. Craig Venter Institute, La Jolla, CA, United States., Bonch-Osmolovskaya EA; Winogradsky Institute of Microbiology, FRC Biotechnology Russian Academy of Sciences, Moscow, Russia.; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 Jan 11; Vol. 11, pp. 597818. Date of Electronic Publication: 2021 Jan 11 (Print Publication: 2020).
DOI:	10.3389/fmicb.2020.597818
Abstrakt:	Biogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pathways is sparse. Here, we describe EET channels in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens that drive exoelectrogenesis and rapid conversion of amorphous mineral ferrihydrite to large magnetite crystals. Microscopic studies indicated biocontrolled formation of unusual formicary-like ultrastructure of the magnetite crystals and revealed active colonization of anodes in bioelectrochemical systems (BESs) by C. ferrireducens . The internal structure of micron-scale biogenic magnetite crystals is reported for the first time. Genome analysis and expression profiling revealed three constitutive c -type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, sharing insignificant homology with previously described EET-related cytochromes thus representing novel determinants of EET. Our studies identify these cytochromes as extracellular and reveal potentially novel mechanisms of cell-to-mineral interactions in thermal environments. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Gavrilov, Zavarzina, Elizarov, Tikhonova, Dergousova, Popov, Lloyd, Knight, El-Naggar, Pirbadian, Leung, Robb, Zakhartsev, Bretschger and Bonch-Osmolovskaya.)
Databáze:	MEDLINE
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