Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens.

Autor:	Menezes-Neto A; Fundação Oswaldo Cruz-Fiocruz, Instituto Aggeu Magalhães, Recife, PE, Brasil., Castilho MDC; Fundação de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus, AM, Brasil., Calvet GA; Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro, RJ, Brasil., Kara EO; World Health Organization, Geneva, Switzerland., Bôtto-Menezes CHA; Universidade do Estado do Amazonas, Manaus, AM, Brasil., Lacerda MVG; Fundação Oswaldo Cruz-Fiocruz, Manaus, AM, Brasil., Pereira GFM; Ministério da Saúde, Secretaria de Vigilância em Saúde, Departamento de Doenças de Condições Crônicas e Infecções Sexualmente Transmissíveis, Brasília, DF, Brasil., Giozza SP; Ministério da Saúde, Secretaria de Vigilância em Saúde, Departamento de Doenças de Condições Crônicas e Infecções Sexualmente Transmissíveis, Brasília, DF, Brasil., Bermudez XPD; Organização Pan Americana de Saúde/Organização Mundial da Saúde, Universidade de Brasília, Departamento de Saúde Pública, Brasília, DF, Brasil., Lima NS; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Modjarrad K; Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Broutet N; World Health Organization, Geneva, Switzerland., de Filippis AMB; Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Franca RFO; Fundação Oswaldo Cruz-Fiocruz, Instituto Aggeu Magalhães, Recife, PE, Brasil.
Jazyk:	angličtina
Zdroj:	Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2021 Jan 25; Vol. 115, pp. e200339. Date of Electronic Publication: 2021 Jan 25 (Print Publication: 2021).
DOI:	10.1590/0074-02760200339
Abstrakt:	We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands.
Databáze:	MEDLINE
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