Discovery of melanin-concentrating hormone receptor 1 in brown adipose tissue.

Autor: Philippe C; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria.; Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria., Klebermass EM; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria.; Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria., Balber T; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria.; Ludwig Boltzmann Institute Applied Diagnostics, Vienna, Austria., Kulterer OC; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria.; Division of Endocrinology and Metabolism, Department of Medicine III, Medical University of Vienna, Vienna, Austria., Zeilinger M; Faculty of Engineering, University of Applied Sciences Wiener Neustadt, Wiener Neustadt, Austria., Egger G; Ludwig Boltzmann Institute Applied Diagnostics, Vienna, Austria.; Department of Pathology, Medical University of Vienna, Vienna, Austria., Dumanic M; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria., Herz CT; Division of Endocrinology and Metabolism, Department of Medicine III, Medical University of Vienna, Vienna, Austria., Kiefer FW; Division of Endocrinology and Metabolism, Department of Medicine III, Medical University of Vienna, Vienna, Austria., Scheuba C; Division of General Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria., Scherer T; Division of Endocrinology and Metabolism, Department of Medicine III, Medical University of Vienna, Vienna, Austria., Fürnsinn C; Division of Endocrinology and Metabolism, Department of Medicine III, Medical University of Vienna, Vienna, Austria., Vraka C; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria., Pallitsch K; Institute of Organic Chemistry, University of Vienna, Vienna, Austria., Spreitzer H; Department of Pharmaceutical Chemistry, University of Vienna, Vienna, Austria., Wadsak W; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria.; Center for Biomarker Research in Medicine - CBmed GmbH, Graz, Austria., Viernstein H; Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria., Hacker M; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria., Mitterhauser M; Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria.; Ludwig Boltzmann Institute Applied Diagnostics, Vienna, Austria.
Jazyk: angličtina
Zdroj: Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 2021 Jun; Vol. 1494 (1), pp. 70-86. Date of Electronic Publication: 2021 Jan 27.
DOI: 10.1111/nyas.14563
Abstrakt: Although extensive research on brown adipose tissue (BAT) has stimulated optimism in the battle against obesity and diabetes, BAT physiology and organ crosstalk are not fully understood. Besides BAT, melanin-concentrating hormone (MCH) and its receptor (MCHR1) play an important role in energy homeostasis. Because of the link between hypothalamic MCH neurons and sympathetic BAT activation via β-adrenoceptors, we investigated the expression and physiological role of the MCHR1 in BAT. MCHR1 was detected in rodent and human BAT with RT-qPCR and western blot analyses. In vivo imaging in rats used the glucose analog [ 18 F]FDG and the MCHR1-tracer [ 11 C]SNAP-7941. We found that the β3-adrenoceptor (ADRB3) agonist CL316,243 increased [ 11 C]SNAP-7941 uptake in BAT. Additionally, a pharmacological concentration of SNAP-7941-a low-affinity ADRB3 ligand-stimulated [ 18 F]FDG uptake, reflecting BAT activation. In cultured human adipocytes, CL316,243 induced MCHR1 expression, further supporting a direct interaction between MCHR1 and ADRB3. These findings characterized MCHR1 expression in rodent and human BAT for the first time, including in vitro and in vivo data demonstrating a link between MCHR1 and the β3-adrenergic system. The presence of MCHR1 in BAT emphasizes the role of BAT in energy homeostasis and may help uncover treatment approaches for obesity.
(© 2021 The Authors. Annals of the New York Academy of Sciences published by Wiley Periodicals LLC on behalf of New York Academy of Sciences.)
Databáze: MEDLINE
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