A Association of MTHFR C677T and MTRR A66G Gene Polymorphisms with Iranian Male Infertility and Its Effect on Seminal Folate and Vitamin B12.
| Autor: | Raigani M; Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.; Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.Email: m_raigani@pasteur.ac.ir., Lakpour N; Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran., Soleimani M; Department of Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran., Johari B; Department of Medical Biotechnology and Nanotechnology, School of Medicine, Zanjan University of Medical Science, Zanjan, Iran., Sadeghi MR; Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Email:sadeghi@avicenna.ac.ir. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of fertility & sterility [Int J Fertil Steril] 2021 Jan; Vol. 15 (1), pp. 20-25. Date of Electronic Publication: 2021 Jan 19. |
| DOI: | 10.22074/ijfs.2021.6155 |
| Abstrakt: | Background: The relation between key enzymes in regulation of folate metabolism and male infertility is the subject of numerous studies. We aimed to determine whether 5, 10-methylenetetrahydrofolate reductase ( MTHFR ) C677T and methionine synthase reductase ( MTRR ) A66G genotypes are associated with male infertility in Iranian men and to evaluate its effect on seminal levels of folate and vitamin B12. Materials and Methods: In this randomized clinical trail study, semen and peripheral blood samples were collected from 254 men with oligoasthenoteratozoospermia (OAT) and 77 normozoospermic men who attended Avicenna infertility clinic. Single nucleotide polymorphism (SNP) analysis was carried out in genomic DNA by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method for MTHFR C677T and MTRR A66G gene polymorphisms. Results: In MTHFR C677T, our founding showed that T carrier was conversely lower in OAT than normozoospermic men (χ 2 -test=7.245, P=0.02) whereas in MTRR A66G, A and G carrier showed no significant difference between the two groups (χ 2 -test=1.079, P=0.53). The concentration of seminal folate was not different between normozoospermic (18.83 ± 17.1 ng/ ml) and OAT (16.96 ± 14.2 ng/ml) men (P=0.47). The concentration of vitamin B12 was slightly higher in normozospermic men (522.6 ± 388.1 pg/ml) compared to OAT men (412.9 ± 303.6 pg/ml, P=0.058). Conclusion: The MTHFR C677T and MTRR A66G have no effect on the concentrations of seminal folate and vitamin B12. The present study showed that two SNPs of MTRR A66G and MTHFR C677T cannot be seen as a risk factor for male factor subfertility. Competing Interests: The authors declare no conflicts of interest. (Copyright© by Royan Institute. All rights reserved.) |
| Databáze: | MEDLINE |
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