| Autor: |
Zdouc MM; Naicons Srl., Viale Ortles 22/4, 20139 Milano, Italy.; Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Iorio M; Naicons Srl., Viale Ortles 22/4, 20139 Milano, Italy., Maffioli SI; Naicons Srl., Viale Ortles 22/4, 20139 Milano, Italy., Crüsemann M; Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität, Nußallee 6, 53115 Bonn, Germany., Donadio S; Naicons Srl., Viale Ortles 22/4, 20139 Milano, Italy., Sosio M; Naicons Srl., Viale Ortles 22/4, 20139 Milano, Italy. |
| Abstrakt: |
Despite an excellent track record, microbial drug discovery suffers from high rates of rediscovery. Better workflows for the rapid investigation of complex extracts are needed to increase throughput and to allow early prioritization of samples. In addition, systematic characterization of poorly explored strains is seldomly performed. Here, we report a metabolomic study of 72 isolates belonging to the rare actinomycete genus Planomonospora , using a workflow of commonly used open access tools to investigate its secondary metabolites. The results reveal a correlation of chemical diversity and strain phylogeny, with classes of metabolites exclusive to certain phylogroups. We were able to identify previously reported Planomonospora metabolites, including the ureylene-containing oligopeptide antipain, the thiopeptide siomycin including new congeners, and the ribosomally synthesized peptides sphaericin and lantibiotic 97518. In addition, we found that Planomonospora strains can produce the siderophore desferrioxamine or a salinichelin-like peptide. Analysis of the genomes of three newly sequenced strains led to the detection of 59 gene cluster families, of which three were connected to products found by LC-MS/MS profiling. This study demonstrates the value of metabolomic studies to investigate poorly explored taxa and provides a first picture of the biosynthetic capabilities of the genus Planomonospora . |
