An Alveolata secretory machinery adapted to parasite host cell invasion.

Autor: Aquilini E; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Cova MM; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Mageswaran SK; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Dos Santos Pacheco N; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Sparvoli D; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France.; Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA., Penarete-Vargas DM; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Najm R; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Graindorge A; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Suarez C; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Maynadier M; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Berry-Sterkers L; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Urbach S; Institut de Génomique Fonctionnelle (IGF), Université de Montpellier, CNRS and INSERM, Montpellier, France., Fahy PR; Pôle Facultaire de Microscopie Ultrastructurale, Geneva, Switzerland., Guérin AN; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA., Striepen B; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA., Dubremetz JF; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France., Chang YW; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Turkewitz AP; Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA., Lebrun M; Laboratory of Pathogen Host Interactions UMR 5235, Université de Montpellier and CNRS, Montpellier, France. mylebrun@univ-montp2.fr.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2021 Apr; Vol. 6 (4), pp. 425-434. Date of Electronic Publication: 2021 Jan 25.
DOI: 10.1038/s41564-020-00854-z
Abstrakt: Apicomplexa are unicellular eukaryotes and obligate intracellular parasites, including Plasmodium (the causative agent of malaria) and Toxoplasma (one of the most widespread zoonotic pathogens). Rhoptries, one of their specialized secretory organelles, undergo regulated exocytosis during invasion 1 . Rhoptry proteins are injected directly into the host cell to support invasion and subversion of host immune function 2 . The mechanism by which they are discharged is unclear and appears distinct from those in bacteria, yeast, animals and plants. Here, we show that rhoptry secretion in Apicomplexa shares structural and genetic elements with the exocytic machinery of ciliates, their free-living relatives. Rhoptry exocytosis depends on intramembranous particles in the shape of a rosette embedded into the plasma membrane of the parasite apex. Formation of this rosette requires multiple non-discharge (Nd) proteins conserved and restricted to Ciliata, Dinoflagellata and Apicomplexa that together constitute the superphylum Alveolata. We identified Nd6 at the site of exocytosis in association with an apical vesicle. Sandwiched between the rosette and the tip of the rhoptry, this vesicle appears as a central element of the rhoptry secretion machine. Our results describe a conserved secretion system that was adapted to provide defence for free-living unicellular eukaryotes and host cell injection in intracellular parasites.
Databáze: MEDLINE
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