Reinforcement of Colonic Anastomosis with Improved Ultrafine Nanofibrous Patch: Experiment on Pig.

Autor:	Rosendorf J; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic.; Department of Surgery, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic., Klicova M; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, 460 01 Liberec, Czech Republic., Cervenkova L; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic., Horakova J; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, 460 01 Liberec, Czech Republic., Klapstova A; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, 460 01 Liberec, Czech Republic., Hosek P; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic., Palek R; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic.; Department of Surgery, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic., Sevcik J; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic., Polak R; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic.; Department of Surgery, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic., Treska V; Department of Surgery, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic., Chvojka J; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, 460 01 Liberec, Czech Republic., Liska V; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic.; Department of Surgery, Faculty of Medicine in Pilsen, Charles University, 301 00 Pilsen, Czech Republic.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2021 Jan 21; Vol. 9 (2). Date of Electronic Publication: 2021 Jan 21.
DOI:	10.3390/biomedicines9020102
Abstrakt:	Anastomotic leakage is a dreadful complication in colorectal surgery. It has a negative impact on postoperative mortality, long term life quality and oncological results. Nanofibrous polycaprolactone materials have shown pro-healing properties in various applications before. Our team developed several versions of these for healing support of colorectal anastomoses with promising results in previous years. In this study, we developed highly porous biocompatible polycaprolactone nanofibrous patches. We constructed a defective anastomosis on the large intestine of 16 pigs, covered the anastomoses with the patch in 8 animals (Experimental group) and left the rest uncovered (Control group). After 21 days of observation we evaluated postoperative changes, signs of leakage and other complications. The samples were assessed histologically according to standardized protocols. The material was easy to work with. All animals survived with no major complication. There were no differences in intestinal wall integrity between the groups and there were no signs of anastomotic leakage in any animal. The levels of collagen were significantly higher in the Experimental group, which we consider to be an indirect sign of higher mechanical strength. The material shall be further perfected in the future and possibly combined with active molecules to specifically influence the healing process.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu