Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori .
| Autor: | Antani JD; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, United States., Sumali AX; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, United States., Lele TP; Department of Biomedical Engineering, Texas A&M University, College Station, TX 77840, College Station, TX 77840, United States.; Department of Translational Medical Sciences, Texas A&M University, Houston, TX 77030, United States., Lele PP; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, United States. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | ELife [Elife] 2021 Jan 25; Vol. 10. Date of Electronic Publication: 2021 Jan 25. |
| DOI: | 10.7554/eLife.63936 |
| Abstrakt: | The canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our understanding of chemotaxis in this species. Here, we determined that H. pylori swim faster (slower) whenever their flagella rotate counterclockwise (clockwise) by analyzing their hydrodynamic interactions with bounding surfaces. This asymmetry in swimming helped quantify the rotational bias. Upon exposure to a chemo-attractant, the bias decreased and the cells tended to swim exclusively in the faster mode. In the absence of a key chemotaxis protein, CheY, the bias was zero. The relationship between the reversal frequency and the rotational bias was unimodal. Thus, H. pylori 's chemotaxis network appears to modulate the probability of clockwise rotation in otherwise counterclockwise-rotating flagella, similar to the canonical network. Competing Interests: JA, AS, TL, PL No competing interests declared (© 2021, Antani et al.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|