Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.

Autor:	Wong P; Department of Medicine, Division of Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA., Wagner JA; Department of Medicine, Division of Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA., Berrien-Elliott MM; Department of Medicine, Division of Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA., Schappe T; Department of Medicine, Division of Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA., Fehniger TA; Department of Medicine, Division of Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2021 Jan 12; Vol. 2 (1), pp. 100262. Date of Electronic Publication: 2021 Jan 12 (Print Publication: 2021).
DOI:	10.1016/j.xpro.2020.100262
Abstrakt:	Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells' ability to kill target cells ex vivo . Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol details specifically an ex vivo cytotoxicity assay using primary murine NK cells enriched from splenocytes that were activated in vivo with poly(I:C). For complete details on the use and execution of this protocol, please refer to Wagner et al. (2020). Competing Interests: The authors declare no competing interests (© 2020.)
Databáze:	MEDLINE
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