Effects of Ibrutinib on biophysical parameters of platelet in patients with chronic lymphocytic leukaemia.

Autor:	Popov VM; Hematology Department, Colentina Clinical Hospital 19-21 Stefan cel Mare Road, Bucharest 020125, Romania., Matei CO; Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania.; Excellence Centre for Research in Biophysics and Cellular Biotechnology, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania., Omer M; Hematology Department, Colentina Clinical Hospital 19-21 Stefan cel Mare Road, Bucharest 020125, Romania., Onisai M; Hematology Department, University Emergency Hospital 169 Splaiul Independentei, Bucharest 050098, Romania., Matei MB; Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania.; Excellence Centre for Research in Biophysics and Cellular Biotechnology, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania., Savopol T; Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania.; Excellence Centre for Research in Biophysics and Cellular Biotechnology, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania., Bumbea H; Hematology Department, University Emergency Hospital 169 Splaiul Independentei, Bucharest 050098, Romania., Moisescu MG; Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania.; Excellence Centre for Research in Biophysics and Cellular Biotechnology, Carol Davila University of Medicine and Pharmacy 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania.
Jazyk:	angličtina
Zdroj:	American journal of blood research [Am J Blood Res] 2020 Dec 15; Vol. 10 (6), pp. 311-319. Date of Electronic Publication: 2020 Dec 15 (Print Publication: 2020).
Abstrakt:	Patients with chronic lymphocytic leukemia (CLL) treated with Ibrutinib often present hemorrhagic complications. Platelets dysfunction is well documented by aggregometry and flow cytometry, but the mechanisms by which Ibrutinib treatment influences the platelets status is yet to be evaluated. The aim of this study is to identify platelet membrane parameters in chronic lymphocytic leukemia (CLL) that could be altered by Ibrutinib administration. In this paper we propose a set of fluorescence measurements of the following parameters: membrane fluidity, resting membrane potential, and reactive oxygen species production of platelets suspensions obtained from CLL patients treated or not with Ibrutinib as markers for platelets status in this pathological situation. Platelets from CLL patients treated with Ibrutinib have higher membrane fluidity, lower resting membrane potential and higher level of reactive oxygen species production compared to the untreated CLL patients. These patients are also presenting higher membrane fluidity and lower resting membrane potential compared to healthy volunteers. Competing Interests: None. (AJBR Copyright © 2020.)
Databáze:	MEDLINE
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