Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves.

Autor: Sebola TE; Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, Johannesburg, South Africa., Uche-Okereafor NC; Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, Johannesburg, South Africa., Mekuto L; Department of Chemical Engineering, Faculty of Engineering and the Built Environment, University of Johannesburg, Doornfontein Campus, Johannesburg, South Africa., Makatini MM; Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Johannesburg, South Africa., Green E; Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, Johannesburg, South Africa., Mavumengwana V; DST-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg Campus, Cape Town, South Africa.
Jazyk: angličtina
Zdroj: International journal of microbiology [Int J Microbiol] 2020 Dec 10; Vol. 2020, pp. 8839490. Date of Electronic Publication: 2020 Dec 10 (Print Publication: 2020).
DOI: 10.1155/2020/8839490
Abstrakt: This study isolated and identified endophytic bacteria from the leaves of Crinum macowanii and investigated the potential of the bacterial endophyte extracts as antibacterial and anticancer agents and their subsequent secondary metabolites. Ethyl acetate extracts from the endophytes and the leaves (methanol: dichloromethane (1 : 1)) were used for antibacterial activity against selected pathogenic bacterial strains by using the broth microdilution method. The anticancer activity against the U87MG glioblastoma and A549 lung carcinoma cells was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Bacterial endophytes that were successfully isolated from C. macowanii leaves include Raoultella ornithinolytica , Acinetobacter guillouiae , Pseudomonas sp., Pseudomonas palleroniana , Pseudomonas putida , Bacillus safensis , Enterobacter asburiae , Pseudomonas cichorii , and Arthrobacter pascens . Pseudomonas cichorii exhibited broad antibacterial activity against both Gram-negative and Gram-positive pathogenic bacteria while Arthrobacter pascens displayed the least MIC of 0.0625 mg/mL. Bacillus safensis crude extracts were the only sample that showed notable cell reduction of 50% against A549 lung carcinoma cells at a concentration of 100  μ g/mL. Metabolite profiling of Bacillus safensis , Pseudomonas cichorii , and Arthrobacter pascens crude extracts revealed the presence of known antibacterial and/or anticancer agents such as lycorine (1), angustine (2), crinamidine (3), vasicinol (4), and powelline. It can be concluded that the crude bacterial endophyte extracts obtained from C. macowanii leaves can biosynthesize bioactive compounds and can be bioprospected for medical application into antibacterial and anticancer agents.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2020 Tendani E. Sebola et al.)
Databáze: MEDLINE
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