Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration.

Autor:	McDonough A; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, United States., Elsen GE; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, United States., Daza RM; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, United States.; Department of Pathology, University of California, San Diego, CA, United States., Bachleda AR; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, United States., Pizzo D; Department of Pathology, University of California, San Diego, CA, United States., DelleTorri OM; California Institute for Regenerative Medicine, California State University San Marcos, San Marcos, CA, United States., Hevner RF; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, United States.; Department of Pathology, University of California, San Diego, CA, United States.; Department of Neurological Surgery, University of Washington, Seattle, WA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in neuroscience [Front Neurosci] 2021 Jan 08; Vol. 14, pp. 598548. Date of Electronic Publication: 2021 Jan 08 (Print Publication: 2020).
DOI:	10.3389/fnins.2020.598548
Abstrakt:	Previous studies demonstrated specific expression of transcription factor Tbr2 in unipolar brush cells (UBCs) of the cerebellum during development and adulthood. To further study UBCs and the role of Tbr2 in their development we examined UBC morphology in transgenic mouse lines (reporter and lineage tracer) and also examined the effects of Tbr2 deficiency in Tbr2 (MGI: Eomes ) conditional knock-out (cKO) mice. In Tbr2 reporter and lineage tracer cerebellum, UBCs exhibited more complex morphologies than previously reported including multiple dendrites, bifurcating dendrites, and up to four dendritic brushes. We propose that "dendritic brush cells" (DBCs) may be a more apt nomenclature. In Tbr2 cKO cerebellum, mature UBCs were completely absent. Migration of UBC precursors from rhombic lip to cerebellar cortex and other nuclei was impaired in Tbr2 cKO mice. Our results indicate that UBC migration and differentiation are sensitive to Tbr2 deficiency. To investigate whether UBCs develop similarly in humans as in rodents, we studied Tbr2 expression in mid-gestational human cerebellum. Remarkably, Tbr2 + UBC precursors migrate along the same pathways in humans as in rodent cerebellum and disperse to create the same "fountain-like" appearance characteristic of UBCs exiting the rhombic lip. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 McDonough, Elsen, Daza, Bachleda, Pizzo, DelleTorri and Hevner.)
Databáze:	MEDLINE
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