Lack of viable severe acute respiratory coronavirus virus 2 (SARS-CoV-2) among PCR-positive air samples from hospital rooms and community isolation facilities.

Autor:	Ong SWX; National Centre for Infectious Diseases, Singapore.; Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore., Tan YK; DSO National Laboratories, Singapore., Coleman KK; Duke-NUS Medical School, National University of Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Tan BH; DSO National Laboratories, Singapore., Leo YS; National Centre for Infectious Diseases, Singapore.; Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore.; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore., Wang DL; DSO National Laboratories, Singapore., Ng CG; DSO National Laboratories, Singapore., Ng OT; National Centre for Infectious Diseases, Singapore.; Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore., Wong MSY; DSO National Laboratories, Singapore., Marimuthu K; National Centre for Infectious Diseases, Singapore.; Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore.; Infection Prevention and Control Office, Woodlands Health Campus, Singapore.
Jazyk:	angličtina
Zdroj:	Infection control and hospital epidemiology [Infect Control Hosp Epidemiol] 2021 Nov; Vol. 42 (11), pp. 1327-1332. Date of Electronic Publication: 2021 Jan 25.
DOI:	10.1017/ice.2021.8
Abstrakt:	Background: Understanding the extent of aerosol-based transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important for tailoring interventions for control of the coronavirus disease 2019 (COVID-19) pandemic. Multiple studies have reported the detection of SARS-CoV-2 nucleic acid in air samples, but only one study has successfully recovered viable virus, although it is limited by its small sample size. Objective: We aimed to determine the extent of shedding of viable SARS-CoV-2 in respiratory aerosols from COVID-19 patients. Methods: In this observational air sampling study, air samples from airborne-infection isolation rooms (AIIRs) and a community isolation facility (CIF) housing COVID-19 patients were collected using a water vapor condensation method into liquid collection media. Samples were tested for presence of SARS-CoV-2 nucleic acid using quantitative real-time polymerase chain reaction (qRT-PCR), and qRT-PCR-positive samples were tested for viability using viral culture. Results: Samples from 6 (50%) of the 12 sampling cycles in hospital rooms were positive for SARS-CoV-2 RNA, including aerosols ranging from <1 µm to >4 µm in diameter. Of 9 samples from the CIF, 1 was positive via qRT-PCR. Viral RNA concentrations ranged from 179 to 2,738 ORF1ab gene copies per cubic meter of air. Virus cultures were negative after 4 blind passages. Conclusion: Although SARS-CoV-2 is readily captured in aerosols, virus culture remains challenging despite optimized sampling methodologies to preserve virus viability. Further studies on aerosol-based transmission and control of SARS-CoV-2 are needed.
Databáze:	MEDLINE
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