The viability of human cells irradiated with 470-nm light at various radiant energies in vitro.

Autor: Bumah VV; Department of Chemistry and Biochemistry, College of Sciences, San Diego State University, San Diego, CA, 92182, USA.; College of Health and Human Services, San Diego State University, San Diego, CA, USA., Masson-Meyers DS; College of Health and Human Services, San Diego State University, San Diego, CA, USA.; Marquette University School of Dentistry, Milwaukee, WI, USA., Awosika O; Klingler College of Arts and Sciences, Marquette University, Milwaukee, WI, USA., Zacharias S; Homestead High School, Mequon, WI, USA., Enwemeka CS; College of Health and Human Services, San Diego State University, San Diego, CA, USA. Enwemeka@sdsu.edu.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2021 Oct; Vol. 36 (8), pp. 1661-1670. Date of Electronic Publication: 2021 Jan 24.
DOI: 10.1007/s10103-021-03250-z
Abstrakt: Blue light is known to be antimicrobial, but its effect on normal cutaneous and subcutaneous cells remains unclear. Therefore, we studied the effect of 470-nm light on the viability of adult and neonatal human dermal fibroblasts, Jurkat T-cells, and THP-1 monocytes in vitro. Each culture was irradiated with 0, 3, 55, or 110 J/cm 2 of 470-nm light and subjected to trypan blue assay to ascertain viability. Further, MTT, neutral red, and fluorescence assays of fibroblasts were performed, and cell morphology visualized using bright field and fluorescence microscopy. At each dose and in each of the four cell lines, there was no significant difference in cell concentration between irradiated and non-irradiated cultures, even though irradiation with 55 J/cm 2 or 110 J/cm 2 slightly decreased cell count. Light microscopy showed progressive morphological changes in the fibroblasts as energy fluence increased from 55 to 110 J/cm 2 . Irradiation at 3 J/cm 2 produced a slight but non-significant increase in the viability of Jurkat T-cells and THP-1 monocytes. In contrast, at 110 J/cm 2 radiant exposure, irradiation slightly decreased the viability of all four cells. While 3 J/cm 2 appears stimulatory, our finding that 110 J/cm 2 produces a slight decrease in viability and engenders morphological changes in fibroblasts, suggesting that such high doses should be avoided in blue light treatments.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd. part of Springer Nature.)
Databáze: MEDLINE
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