Cloning, expression and immunological characterisation of Coc n 1, the first major allergen from Coconut pollen.

Autor: Saha B; Division of Plant Biology, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India; Chemistry Research Laboratory, 12 Mansfield Road, OX4 4TG, Oxford, United Kingdom. Electronic address: bodhisattwasaha@gmail.com., Karmakar B; Division of Plant Biology, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India., Bhattacharya SG; Division of Plant Biology, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India. Electronic address: swati@jcbose.ac.in.
Jazyk: angličtina
Zdroj: Molecular immunology [Mol Immunol] 2021 Mar; Vol. 131, pp. 33-43. Date of Electronic Publication: 2021 Jan 21.
DOI: 10.1016/j.molimm.2020.12.026
Abstrakt: Coconut pollen has been documented to be a major contributor to the aeroallergen load in India, causing respiratory allergy in a large cohort of susceptible individuals. Here, we report the identification of the first major allergen from Coconut pollen, Coc n 1. The full-length sequence of the allergen was determined from previously identified peptides and overexpressed in E. coli. Recombinant Coc n 1 folded into a trimer and was found to possess allergenicity equivalent to its natural counterpart. Proteolytic processing of Coc n 1 led to the formation of an immunodominant ∼20 kDa C-terminal subunit and the site of cleavage was determined by amino acid microsequencing. Five linear IgE binding epitopes were predicted and mapped on the homology modelled structure of Coc n 1. Amongst three immunodominant epitopes, two were present towards the C-terminal end. Coc n 1 was found to belong to the highly diverse cupin superfamily and mimics its structure with known 7S globulin or vicilin allergens but lacks sequence similarity. Using sequence similarity networks, Coc n 1 clustered as a separate group containing unannotated cupin domain proteins and did not include known vicilin allergens except Gly m Bd 28 kDa, a Soybean major allergen. 7S globulins are major storage proteins and food allergens, but presence of such protein in pollen grains is reported for the first time. Further study on Coc n 1 may provide insights into its function in pollen grains and also in the development of immunotherapy to Coconut pollen allergy.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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