Boosting the antibacterial activity of chitosan-gold nanoparticles against antibiotic-resistant bacteria by Punicagranatum L. extract.

Autor: Hussein MAM; Clinic of Dermatology, University Hospital of Aachen, Aachen 52074, Germany; Department of Pharmacology, Medical Research Division, National Research Center, Dokki, Cairo 12622, Egypt. Electronic address: almohammadeymr2023@gmail.com., Grinholc M; Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, Gdansk, Poland. Electronic address: mariusz.grinholc@biotech.ug.edu.pl., Dena ASA; Nanomedicine Laboratory, Center for Materials Science (CMS), Zewail City of Science and Technology, 6(th) of October, Giza 12578, Egypt. Electronic address: ahmed_said5899@yahoo.com., El-Sherbiny IM; Nanomedicine Laboratory, Center for Materials Science (CMS), Zewail City of Science and Technology, 6(th) of October, Giza 12578, Egypt. Electronic address: ielsherbiny@zewailcity.edu.eg., Megahed M; Clinic of Dermatology, University Hospital of Aachen, Aachen 52074, Germany. Electronic address: mmegahed@ukaachen.de.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2021 Mar 15; Vol. 256, pp. 117498. Date of Electronic Publication: 2020 Dec 14.
DOI: 10.1016/j.carbpol.2020.117498
Abstrakt: The present work describes the synthesis of a new series of chitosan-gold hybrid nanoparticles (CS-AuNPs) for the delivery of Punicagranatum L. extract (PE). It proposes CS and PE as reducing agents for gold ions in aqueous solution. The effect of PE on the physicochemical properties of the CS-AuNPs was investigated with UV spectroscopy, DLS, DSC, XRD, FTIR, SEM/EDX and TEM. Interestingly, about 50 % reduction in size was observed with using PE alone for gold reduction. The ζ-potential of CS-AuNPs was shifted from +53.1 ± 6.7 mV to 31.0 ± 6.0 mV upon conjugation of the negatively-charged PE polyphenols. The developed PE-conjugated CS-AuNPs exhibited higher stability at different pH values. About 87 % of the loaded PE was released from the NPs over 24 h. The antibacterial activity of CS-PE-AuNPs displayed a synergetic affect against methicillin-resistant S. aureus with MIC and MBC values of 15.6 and 62.5 μg/mL, respectively.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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