Methylation of the PTENP1 pseudogene as potential epigenetic marker of age-related changes in human endometrium.

Autor: Kovalenko TF; Laboratory of membrane bioenergetics, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow, Russia., Morozova KV; Department of Obstetrics and Gynecology, Pirogov Russian National Research Medical University, Moscow, Russia., Pavlyukov MS; Laboratory of membrane bioenergetics, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow, Russia., Anufrieva KS; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Laboratory of Cell Biology, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Moscow Institute of Physics and Technology (State University), Moscow Region, Russia., Bobrov MY; Laboratory of Molecular Pathophysiology, Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russian Federation, Moscow, Russia., Gamisoniya AM; Laboratory of Molecular Pathophysiology, Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russian Federation, Moscow, Russia.; Laboratory of oxylipins, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow, Russia., Ozolinya LA; Department of Obstetrics and Gynecology, Pirogov Russian National Research Medical University, Moscow, Russia., Dobrokhotova YE; Department of Obstetrics and Gynecology, Pirogov Russian National Research Medical University, Moscow, Russia., Shakhparonov MI; Laboratory of membrane bioenergetics, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow, Russia., Patrushev LI; Educational & scientific center, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow, Russia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Jan 22; Vol. 16 (1), pp. e0243093. Date of Electronic Publication: 2021 Jan 22 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0243093
Abstrakt: The processed pseudogene PTENP1 is involved in the regulation of the expression of the PTEN and acts as a tumor suppressor in many types of malignances. In our previous study we showed that PTENP1 methylation is present not only in tumor, but also in normal endometrium tissues of women over 45 years old. Here we used methylation-specific PCR to analyze methylation status of CpG island located near promoter region of PTENP1 in malignant and non-malignant endometrium tissues collected from 236 women of different age groups. To confirm our results, we also analyzed RNA sequencing and microarray data from 431 women with endometrial cancer from TCGA database. We demonstrated that methylation of PTENP1 is significantly increased in older patients. We also found an age-dependent increase in the level of PTENP1 expression in endometrial tissue. According to our data, PTENP1 methylation elevates the level of the pseudogene sense transcript. In turn, a high level of this transcript correlates with a more favorable prognosis in endometrial cancer. The data obtained suggested that PTENP1 methylation is associated with age-related changes in normal and hyperplastic endometrial tissues. We assumed that age-related increase in PTENP1 methylation and subsequent elevation of its expression may serve as a protective mechanism aimed to prevent malignant transformation of endometrial tissue in women during the perimenopause, menopause, and postmenopause periods.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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