Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling.

Autor:	Steinfeld N; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.; Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI, USA., Giridharan SSP; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA., Kauffman EJ; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA., Weisman LS; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA. lweisman@umich.edu.; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. lweisman@umich.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2251, pp. 1-17.
DOI:	10.1007/978-1-0716-1142-5_1
Abstrakt:	Phosphoinositide (PPI) lipids are a crucial class of low-abundance signaling molecules that regulate many processes within cells. Methods that enable simultaneous detection of all PPI lipid species provide a wholistic snapshot of the PPI profile of cells, which is critical for probing PPI biology. Here we describe a method for the simultaneous measurement of cellular PPI levels by metabolically labeling yeast or mammalian cells with myo- 3 H-inositol, extracting radiolabeled glycerophosphoinositides, and separating lipid species on an anion exchange column via HPLC.
Databáze:	MEDLINE
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