| Autor: |
Kruse S; Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Türkowsky D; Department Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Birkigt J; Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Matturro B; Water Research Institute, IRSA-CNR, Monterotondo, Rome, Italy., Franke S; Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.; Eurofins Institute Dr. Appelt Leipzig, Leipzig, Germany., Jehmlich N; Department Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., von Bergen M; Department Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.; Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig, Leipzig, Germany., Westermann M; Center for Electron Microscopy of the University Hospital Jena, Jena, Germany., Rossetti S; Water Research Institute, IRSA-CNR, Monterotondo, Rome, Italy., Nijenhuis I; Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Adrian L; Chair of Geobiotechnology, Technische Universität Berlin, Berlin, Germany.; Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Diekert G; Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Goris T; Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Jena, Germany. tobias.goris@uni-jena.de.; German Institute of Human Nutrition, Department Molecular Toxicology, Research Group Intestinal Microbiology, Potsdam-Rehbrücke, Nuthetal, Germany. tobias.goris@uni-jena.de. |
| Abstrakt: |
Microbial communities involving dehalogenating bacteria assist in bioremediation of areas contaminated with halocarbons. To understand molecular interactions between dehalogenating bacteria, we co-cultured Sulfurospirillum multivorans, dechlorinating tetrachloroethene (PCE) to cis-1,2-dichloroethene (cDCE), and Dehalococcoides mccartyi strains BTF08 or 195, dehalogenating PCE to ethene. The co-cultures were cultivated with lactate as electron donor. In co-cultures, the bacterial cells formed aggregates and D. mccartyi established an unusual, barrel-like morphology. An extracellular matrix surrounding bacterial cells in the aggregates enhanced cell-to-cell contact. PCE was dehalogenated to ethene at least three times faster in the co-culture. The dehalogenation was carried out via PceA of S. multivorans, and PteA (a recently described PCE dehalogenase) and VcrA of D. mccartyi BTF08, as supported by protein abundance. The co-culture was not dependent on exogenous hydrogen and acetate, suggesting a syntrophic relationship in which the obligate hydrogen consumer D. mccartyi consumes hydrogen and acetate produced by S. multivorans. The cobamide cofactor of the reductive dehalogenase-mandatory for D. mccartyi-was also produced by S. multivorans. D. mccartyi strain 195 dechlorinated cDCE in the presence of norpseudo-B 12 produced by S. multivorans, but D. mccartyi strain BTF08 depended on an exogenous lower cobamide ligand. This observation is important for bioremediation, since cofactor supply in the environment might be a limiting factor for PCE dehalogenation to ethene, described for D. mccartyi exclusively. The findings from this co-culture give new insights into aggregate formation and the physiology of D. mccartyi within a bacterial community. |
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