Detection of endocrine disrupting chemicals in Danio rerio and Daphnia pulex: Step-one, behavioral screen.

Autor: Alla LNR; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Monshi M; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Siddiqua Z; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Shields J; Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA., Alame K; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Wahls A; Department of Civil and Environmental Engineering, College of Engineering, Wayne State University, Detroit, MI, USA; Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA., Akemann C; Department of Pharmacology, School of Medicine, Wayne State University, Detroit, MI, USA., Meyer D; Department of Pharmacology, School of Medicine, Wayne State University, Detroit, MI, USA., Crofts EJ; Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA., Saad F; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., El-Nachef J; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Antoon M; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Nakhle R; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Hijazi N; Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA., Hamid M; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA., Gurdziel K; Genomics Sciences Core, Wayne State University, Detroit, MI, USA., McElmurry SP; Department of Civil and Environmental Engineering, College of Engineering, Wayne State University, Detroit, MI, USA., Kashian DR; Department of Biological Sciences, College of Liberal Arts, Wayne State University, Detroit, MI, USA., Baker TR; Department of Pharmacology, School of Medicine, Wayne State University, Detroit, MI, USA; Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA., Pitts DK; Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA. Electronic address: pitts@wayne.edu.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2021 May; Vol. 271, pp. 129442. Date of Electronic Publication: 2021 Jan 02.
DOI: 10.1016/j.chemosphere.2020.129442
Abstrakt: Anthropogenic surface and ground water contamination by chemicals is a global problem, and there is an urgent need to develop tools to identify and elucidate biological effects. Contaminants of emerging concern (CECs) are not typically monitored or regulated and those with known or suspected endocrine disrupting potential have been termed endocrine disrupting chemicals (EDCs). Many CECs are known to be neurotoxic (e.g., insecticides) and many are incompletely characterized. Behavioral responses can identify chemicals with neuroactive properties, which can be relevant to EDC mechanisms (e.g., neuroendocrine disturbances). Two freshwater species, Daphnia pulex and Danio rerio, were evaluated for swimming behavior alterations resulting from 24-hr exposure to 9 CECs: triclosan, triclocarban, chlorpyrifos, dieldrin, 4-nonylphenol, bisphenol-A, atrazine, metformin, and estrone. This is the first step in the development of a bioassay for detecting estrogenic and/or anti-androgenic activity with the goal to evaluate complex mixtures of uncharacterized contaminants in water samples. The second step, described in a subsequent report, examines transcriptome alterations following chemical exposure. Significant differences in the swimming behavior response and sensitivity were found across chemicals within a species and across species for a given chemical in this unique optical bioassay system. In the concentration ranges studied, significant behavioral alterations were detected for 6 of 9 CECs for D. pulex and 4 of 9 CECs for D. rerio. These results underscore the utility of this bioassay to identify behavioral effects of sublethal concentrations of CECs before exploration of transcriptomic alterations for EDC detection.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: