Extraction of DNA from micro-tissue for bat species identification.
| Autor: | Mota TFM; Programa de Pós-Graduação em Biologia Comparada, Universidade Estadual de Maringá, Maringá, Brazil., Fabrin TMC; Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, Maringá, Brazil., Gasques LS; Departamento de Ciências Biológicas, Universidade Paranaense - UNIPAR, Umuarama, Brazil., Ortêncio Filho H; Departamento de Ciências, Universidade Estadual de Maringá, Maringá, Brazil., Prioli AJ; Nupélia, Universidade Estadual de Maringá, Maringá, Brazil., Prioli SMAP; Departamento de Biotecnologia, Genética e Biologia Celular/Nupélia, Universidade Estadual de Maringá, Maringá, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Mitochondrial DNA. Part B, Resources [Mitochondrial DNA B Resour] 2018 Jul 10; Vol. 3 (2), pp. 758-762. Date of Electronic Publication: 2018 Jul 10. |
| DOI: | 10.1080/23802359.2018.1484261 |
| Abstrakt: | Bat populations are declining worldwide. Accurate identification is essential to promote species' conservation. However, minimal morphological differences and a high rate of cryptic species make identification difficult, unless voucher specimens are kept, a controversial issue today. The objective of this work was to standardize a method of extracting non-lethal DNA using bats' uropatagium micro-tissue, aiming the molecular identification of species that occur in the region of Maringá PR. The method standardized was efficient, and does not cause serious damage to bats. For future field studies, collection of micro-tissue and morphometry of the specimens will be sufficient for accurate identification. Competing Interests: No potential conflict of interest was reported by the authors. (© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.) |
| Databáze: | MEDLINE |
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