Evaluation of curcumin-loaded polymeric nanocapsules with different coatings in chick embryo model: influence on angiogenesis, teratogenesis and oxidative stress.

Autor: de Carvalho FB; Postgraduate Program in Pharmaceutical Sciences, Federal University of Pampa, Campus Uruguaiana, BR 472, Km 7, Uruguaiana, RS, 97500-970, Brazil.; Pharmacy Course, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil., de Gomes MG; Postgraduate Program in Pharmaceutical Sciences, Federal University of Pampa, Campus Uruguaiana, BR 472, Km 7, Uruguaiana, RS, 97500-970, Brazil. marcelogomesdegomes@hotmail.com., Savall ASP; Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil., Fidelis EM; Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil., Pinton S; Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil., Ribeiro ACF; Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil., Munieweg FR; Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil., Oelke CA; Department of Veterinary Medicine, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil., Haas SE; Postgraduate Program in Pharmaceutical Sciences, Federal University of Pampa, Campus Uruguaiana, BR 472, Km 7, Uruguaiana, RS, 97500-970, Brazil. sandrahaas@unipampa.edu.br.; Postgraduate Program in Biochemistry, Federal University of Pampa, UNIPAMPA, Uruguaiana, Brazil. sandrahaas@unipampa.edu.br.
Jazyk: angličtina
Zdroj: Pharmacological reports : PR [Pharmacol Rep] 2021 Apr; Vol. 73 (2), pp. 563-573. Date of Electronic Publication: 2021 Jan 20.
DOI: 10.1007/s43440-021-00218-2
Abstrakt: Background: Curcumin (CUR) is a bioactive compound with several proven pharmacological properties. However, the major limitation for therapeutic use of CUR is its low bioavailability. In this sense, an alternative to this question is the use of polymeric nanocapsules (NC) as drug/nutraceutical delivery systems. Thus, the aim of current study was to assess the effect of CUR-loaded NC and their different coatings in chick embryo model, evaluating angiogenic, teratogenic and oxidative stress parameters.
Methods: The physicochemical characterization of unloaded and loaded NC with different coatings: (U-NC (P80), U-NC (PEG), U-NC (EUD), U-NC (CS), CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS)) were performed. After 9 days of incubation, eggs were treated (10 mL/kg eggs; via injection) with NC (unloaded and loaded with CUR) and CUR-solution. In sequence, hen's egg test-chorioallantoic membrane (HET-CAM), angiogenic assay, external abnormalities, weight of embryos and oxidative stress markers (TBARS, NPSH, ROS and CAT) were analyzed.
Results: CUR-NC (P80, PEG, EUD and CS) treatments caused antiangiogenic and non-teratogenic effects in chick embryo model. Still, CUR-NC (P80), CUR-NC (PEG), CUR-NC (EUD) and CUR-NC (CS) did not alter markers of oxidative stress (TBARS, NPSH, CAT) studied. Only CUR-NC (EUD) caused increase in ROS levels.
Conclusion: Wherefore, these findings of present study represent a advance in research of drug/nutraceutical delivery systems.
Databáze: MEDLINE
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