Comparative computer-assisted sperm analysis in non-human primates.

Autor: Schmidt JK; Wisconsin National Primate Research Center, Madison, WI, USA., Mean KD; Wisconsin National Primate Research Center, Madison, WI, USA., Dusek BM; Wisconsin National Primate Research Center, Madison, WI, USA., Hinkle HM; Wisconsin National Primate Research Center, Madison, WI, USA., Puntney RC; Wisconsin National Primate Research Center, Madison, WI, USA., Alexander ES; Wisconsin National Primate Research Center, Madison, WI, USA., Malicki KB; Wisconsin National Primate Research Center, Madison, WI, USA., Sneed EL; Wisconsin National Primate Research Center, Madison, WI, USA., Moy AW; Wisconsin National Primate Research Center, Madison, WI, USA., Golos TG; Wisconsin National Primate Research Center, Madison, WI, USA.; Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, USA.; Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA.
Jazyk: angličtina
Zdroj: Journal of medical primatology [J Med Primatol] 2021 Apr; Vol. 50 (2), pp. 108-119. Date of Electronic Publication: 2021 Jan 20.
DOI: 10.1111/jmp.12510
Abstrakt: Background: Biomedical research has recently focused on developing new models of human disease by implementing genome-editing strategies in non-human primates (NHPs) to introduce relevant gene mutations. There is a need to establish objective semen evaluation methods to select sires for in vitro fertilization to perform germline editing in embryos.
Methods: Sperm motility kinematic parameters were evaluated using a computer-assisted semen analysis (CASA) instrument for rhesus macaques (Macaca mulatta), cynomolgus macaques (Macaca fascicularis), and common marmosets (Callithrix jacchus).
Results: Normative sperm kinematic parameters were established, revealing differences between marmosets and macaques. The impact of season on rhesus macaque sperm motility was modest, where changes in sperm motility related to season were dependent on the individual male.
Conclusions: These data provide a baseline of normative kinematic parameters for three captive NHP species, in which implementation of CASA may serve as a tool to evaluate NHP semen quality.
(© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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