| Autor: |
Mendes V; Departamento de Análises Clínicas e Biomedicina, Universidade Estadual do Paraná (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Veiga FF; Departamento de Análises Clínicas e Biomedicina, Universidade Estadual do Paraná (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., de Castro-Hoshino LV; Departamento de Física, Universidade Estadual de Maringá (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Sato F; Departamento de Física, Universidade Estadual de Maringá (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Baesso ML; Departamento de Física, Universidade Estadual de Maringá (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Vesco B; Departamento de Análises Clínicas e Biomedicina, Universidade Estadual do Paraná (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Cruz E; Departamento de Análises Clínicas e Biomedicina, Universidade Estadual do Paraná (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Ferreira ICP; Departamento de Farmácia, Universidade Estadual de Maringá (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Negri M; Departamento de Análises Clínicas e Biomedicina, Universidade Estadual do Paraná (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil., Svidzinski TIE; Departamento de Análises Clínicas e Biomedicina, Universidade Estadual do Paraná (UEM), Avenida Colombo 5790, 87020-900 Maringá-PR, Brazil. |
| Abstrakt: |
We evaluated a hydroalcoholic extract of Sapindus saponaria L. pericarps (ETHOSS), as a candidate to a topical antifungal medicine for onychomycosis. ETHOSS was produced by extracting the crushed fruits in ethanol. The saponin contents were identified and characterized by electrospray ionization mass spectrometry. We measured the in vitro antifungal activity against three dermatophyte fungi, isolated from onychomycosis: Trichophyton rubrum , T. mentagrophytes, and T. interdigitale , using broth microdilution tests. The minimum fungicide concentration of ETHOSS ranged from 195.31 to 781.25 μg/mL. The cytotoxicity of the crude extract was tested on the HeLa cell line, and its ability to permeate into healthy human nails by photoacoustic spectroscopy and Fourier transformation infrared spectrometer (FTIR) spectroscopy by attenuated total reflection. Besides its strong antifungal activity, ETHOSS showed low cytotoxicity in human cells. It was able to permeate and reach the full thickness of the nail in one hour, without the aid of facilitating vehicles, and remained there for at least 24 h. These results suggest that ETHOSS has great potential for treating onychomycosis. |
