Proteins from Rhinella jimi parotoid gland secretion: A comprehensive analytical approach.

Autor: Alexandre LS; Laboratório de Produtos Naturais - LPN, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Braga FMS; Grupo de Estudos em Bioanalítica - GEBIO, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., de Oliveira PK; Grupo de Estudos em Bioanalítica - GEBIO, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Coelho TLS; Grupo de Estudos em Bioanalítica - GEBIO, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Fonseca MG; Federal University of Piauí, Department of Biology, Campus Senador Helvídio Nunes de Barros, Picos, Piauí, Brazil., de Sousa RWR; Laboratory of Experimental Cancerology, Department of Biophysics and Physiology, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Dittz D; Federal University of Piauí, Department of Biochemistry and Pharmacology, Teresina, Piauí, Brazil., de Castro E Sousa JM; Laboratory of Experimental Cancerology, Department of Biophysics and Physiology, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Ferreira PMP; Laboratory of Experimental Cancerology, Department of Biophysics and Physiology, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Dantas C; Laboratório de Química Computacional Inorgânica e Quimiometria - LQCINMETRIA, State University of Maranhão - UEMA, Campus Caxias, 65604-380, Caxias, MA, Brazil., Barbosa HS; Grupo de Estudos em Bioanalítica - GEBIO, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Chaves MH; Laboratório de Produtos Naturais - LPN, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil., Lopes Júnior CA; Grupo de Estudos em Bioanalítica - GEBIO, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil. Electronic address: ciceroalj@ufpi.edu.br., Vieira Júnior GM; Laboratório de Produtos Naturais - LPN, Department of Chemistry, Federal University of Piauí, 64049-550, Teresina, Piauí, Brazil. Electronic address: magela@ufpi.edu.br.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2021 Mar; Vol. 192, pp. 32-39. Date of Electronic Publication: 2021 Jan 16.
DOI: 10.1016/j.toxicon.2021.01.005
Abstrakt: Toad skin secretions are sources of complex mixtures of bioactive compounds, such as proteins and peptides. Rhinella jimi species is a common toad in the Brazilian northeast, considered by only a few known studies. The experimental design was applied to optimize the protein extraction method from R. jimi parotoid gland secretions. The optimum condition was using 100 mmol L -1 Tris-HCl buffer pH 7.2 under vortexing for 5 min. The FTIR analysis combined with PCA revealed high-protein purity of the extracts, confirming the success of the proposed extraction method. The total protein concentration by the Bradford method was 102.4 and 66.5 mg g -1 on toad poisons from Teresina and Picos, respectively. The comparative proteomic analysis using HPLC-SEC-DAD and 1D SDS-PAGE revealed significant differences in protein abundance. HMW biomolecules showed greater abundance in toads from Teresina, while LMW protein species were more abundant in toads from Picos. The significant difference in amphibian proteome can be attributed to the edaphoclimatic conditions of their habitat. The cytotoxicity of the protein extract from Teresina was higher on the tumor cell lines 4T1 and CT26.WT. These new findings are fundamental for future studies the on identity and biological activity of biomolecules from this noble sample.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: