Hmx1 regulates urfh1 expression in the craniofacial region in zebrafish.

Autor: El Fersioui Y; IRO - Institute for Research in Ophthalmology, Sion, Switzerland., Pinton G; IRO - Institute for Research in Ophthalmology, Sion, Switzerland., Allaman-Pillet N; IRO - Institute for Research in Ophthalmology, Sion, Switzerland., Schorderet DF; IRO - Institute for Research in Ophthalmology, Sion, Switzerland.; Faculty of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.; Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Jan 19; Vol. 16 (1), pp. e0245239. Date of Electronic Publication: 2021 Jan 19 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0245239
Abstrakt: H6 family homeobox 1 (HMX1) regulates multiple aspects of craniofacial development as it is widely expressed in the eye, peripheral ganglia and branchial arches. Mutations in HMX1 are linked to an ocular defect termed Oculo-auricular syndrome of Schorderet-Munier-Franceschetti (MIM #612109). We identified UHRF1 as a target of HMX1 during development. UHRF1 and its partner proteins actively regulate chromatin modifications and cellular proliferation. Luciferase assays and in situ hybridization analyses showed that HMX1 exerts a transcriptional inhibitory effect on UHRF1 and a modification of its expression pattern. Overexpression of hmx1 in hsp70-hmx1 zebrafish increased uhrf1 expression in the cranial region, while mutations in the hmx1 dimerization domains reduced uhrf1 expression. Moreover, the expression level of uhrf1 and its partner dnmt1 was increased in the eye field in response to hmx1 overexpression. These results indicate that hmx1 regulates uhrf1 expression and, potentially through regulating the expression of factors involved in DNA methylation, contribute to the development of the craniofacial region of zebrafish.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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