Integrating Microstructured Electrospun Scaffolds in an Open Microfluidic System for in Vitro Studies of Human Patient-Derived Primary Cells.

Autor:	Guida P; Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy., Piscitelli E; Institute of Biomedical Technologies, National Research Council of Italy, via F.lli Cervi 93, 20090 Segrate, Milan, Italy., Marrese M; Institute of Composite and Biomedical Materials, National Research Council of Italy, Mostra D'Oltremare, Pad. 20, viale Kennedy 54, 80125 Naples, Italy., Martino V; Institute of Biomedical Technologies, National Research Council of Italy, via F.lli Cervi 93, 20090 Segrate, Milan, Italy., Cirillo V; Institute of Composite and Biomedical Materials, National Research Council of Italy, Mostra D'Oltremare, Pad. 20, viale Kennedy 54, 80125 Naples, Italy., Guarino V; Institute for Polymers, Composites & Biomaterials, National Research Council of Italy, Mostra d'Oltremare, Pad. 20, viale Kennedy 54, 80125 Naples, Italy., Angeli E; Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy., Cocola C; Institute of Biomedical Technologies, National Research Council of Italy, via F.lli Cervi 93, 20090 Segrate, Milan, Italy.; Consorzio Italbiotech, via Fantoli 15/16, 20138 Milan, Italy., Pelucchi P; Institute of Biomedical Technologies, National Research Council of Italy, via F.lli Cervi 93, 20090 Segrate, Milan, Italy., Repetto L; Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy., Firpo G; Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy., Karnavas T; Columbia University, Department of Genetics & Development, New York, 10032 United States., Gotte M; Department of Gynecology and Obstetrics, Münster University Hospital, 48149 Münster, Germany., Gritzapis A; Department of Breast Cancer Surgery, Hospital 'Agios Savvas', Leoforos Alexandras Avenue, 171, 11522 Athens, Greece., D'Albore M; Institute of Composite and Biomedical Materials, National Research Council of Italy, Mostra D'Oltremare, Pad. 20, viale Kennedy 54, 80125 Naples, Italy., Repetto D; Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy., Pezzuoli D; Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy., Missitzis I; Department of Breast Cancer Surgery, Hospital 'Agios Savvas', Leoforos Alexandras Avenue, 171, 11522 Athens, Greece., Porta G; Department of Medicine and Surgery, University of Insubria, Varese, Italy., Bertalot G; IEO, European Institute of Oncology IRCCS, via G Ripamonti, 435, 20141 Milan, Italy., Bellipanni G; Center for Biotechnology, Sbarro Institute for Research and Molecular Medicine and Department of Biology, Temple University, Philadelphia 19122, United States., Zucchi I; Institute of Biomedical Technologies, National Research Council of Italy, via F.lli Cervi 93, 20090 Segrate, Milan, Italy., Ambrosio L; Institute for Polymers, Composites & Biomaterials, National Research Council of Italy, Mostra d'Oltremare, Pad. 20, viale Kennedy 54, 80125 Naples, Italy., Valbusa U; Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy., Reinbold RA; Institute of Biomedical Technologies, National Research Council of Italy, via F.lli Cervi 93, 20090 Segrate, Milan, Italy.
Jazyk:	angličtina
Zdroj:	ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2020 Jun 08; Vol. 6 (6), pp. 3649-3663. Date of Electronic Publication: 2020 May 04.
DOI:	10.1021/acsbiomaterials.0c00352
Abstrakt:	Recent studies have suggested that microenvironmental stimuli play a significant role in regulating cellular proliferation and migration, as well as in modulating self-renewal and differentiation processes of mammary cells with stem cell (SCs) properties. Recent advances in micro/nanotechnology and biomaterial synthesis/engineering currently enable the fabrication of innovative tissue culture platforms suitable for maintenance and differentiation of SCs in vitro. Here, we report the design and fabrication of an open microfluidic device (OMD) integrating removable poly(ε-caprolactone) (PCL) based electrospun scaffolds, and we demonstrate that the OMD allows investigation of the behavior of human cells during in vitro culture in real time. Electrospun scaffolds with modified surface topography and chemistry can influence attachment, proliferation, and differentiation of mammary SCs and epigenetic mechanisms that maintain luminal cell identity as a function of specific morphological or biochemical cues imparted by tailor-made fiber post-treatments. Meanwhile, the OMD architecture allows control of cell seeding and culture conditions to collect more accurate and informative in vitro assays. In perspective, integrated systems could be tailor-made to mimic specific physiological conditions of the local microenvironment and then analyze the response from screening specific drugs for more effective diagnostics, long-term prognostics, and disease intervention in personalized medicine.
Databáze:	MEDLINE
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