Development of a Microfluidic Droplet-Based Microbioreactor for Microbial Cultivation.

Autor:	Ho CMB; Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia., Sun Q; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia., Teo AJT; Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia., Wibowo D; Centre for Cell Factories and Biopolymers, Griffith Institute for Drug Discovery, Griffith University, Nathan, QLD 4111, Australia., Gao Y; School of Engineering, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia., Zhou J; School of Information and Communication Technology, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia., Huang Y; Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, 100084 Beijing, China., Tan SH; Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Brisbane, QLD 4111, Australia., Zhao CX; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia.
Jazyk:	angličtina
Zdroj:	ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2020 Jun 08; Vol. 6 (6), pp. 3630-3637. Date of Electronic Publication: 2020 May 14.
DOI:	10.1021/acsbiomaterials.0c00292
Abstrakt:	Droplet microfluidics creates new opportunities for microbial engineering. Most microbial cultivations are carried out in bioreactors, which are usually bulky and consume a large amount of reagents and media. In this paper, we propose a microfluidic droplet-based microbioreactor for microbial cultivation. A microfluidic device was designed and fabricated to produce many droplet-based microbioreactors integrated with an AC electric field for the manipulation of these microbioreactors. Droplets encapsulating fluorescent Escherichia coli cells were generated, sorted, and trapped individually in small chambers. Fluorescence intensity was monitored to determine cell growth. An electric field with varying voltages and frequencies manipulates the droplets, simulating an oscillation effect. Initial results showed that electric field does not affect cell growth. A comparison with shake flask showed that a similar standard growth curve is obtained when cultivating at room temperature. This device has the potential for making droplet-based microbioreactors an alternative for microbial engineering research.
Databáze:	MEDLINE
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