Antagonism between germ cell-less and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.

Autor:	Colonnetta MM; Department of Molecular Biology, Princeton University, Princeton, United States., Lym LR; Department of Cell Biology, Emory University School of Medicine, Atlanta, United States., Wilkins L; Department of Molecular Biology, Princeton University, Princeton, United States., Kappes G; Department of Molecular Biology, Princeton University, Princeton, United States., Castro EA; Department of Cell Biology, Emory University School of Medicine, Atlanta, United States., Ryder PV; Department of Cell Biology, Emory University School of Medicine, Atlanta, United States., Schedl P; Department of Molecular Biology, Princeton University, Princeton, United States., Lerit DA; Department of Cell Biology, Emory University School of Medicine, Atlanta, United States., Deshpande G; Department of Molecular Biology, Princeton University, Princeton, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Jan 18; Vol. 10. Date of Electronic Publication: 2021 Jan 18.
DOI:	10.7554/eLife.54346
Abstrakt:	Transcriptional quiescence, an evolutionarily conserved trait, distinguishes the embryonic primordial germ cells (PGCs) from their somatic neighbors. In Drosophila melanogaster , PGCs from embryos maternally compromised for germ cell-less ( gcl ) misexpress somatic genes, possibly resulting in PGC loss. Recent studies documented a requirement for Gcl during proteolytic degradation of the terminal patterning determinant, Torso receptor. Here we demonstrate that the somatic determinant of female fate, Sex-lethal ( Sxl ), is a biologically relevant transcriptional target of Gcl. Underscoring the significance of transcriptional silencing mediated by Gcl, ectopic expression of a degradation-resistant form of Torso ( torso Deg ) can activate Sxl transcription in PGCs, whereas simultaneous loss of torso-like ( tsl ) reinstates the quiescent status of gcl PGCs. Intriguingly, like gcl mutants, embryos derived from mothers expressing torso Deg in the germline display aberrant spreading of pole plasm RNAs, suggesting that mutual antagonism between Gcl and Torso ensures the controlled release of germ-plasm underlying the germline/soma distinction. Competing Interests: MC, LL, LW, GK, EC, PR, PS, DL, GD No competing interests declared (© 2021, Colonnetta et al.)
Databáze:	MEDLINE
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