A long-term in vitro culture of bovine epithelial cells on collagen rafts.

Autor: Peña-Zanoni M; Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina., García DC; Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina.; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina., Roldán-Olarte M; Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina.; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina., Valdecantos PA; Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2021 Apr; Vol. 56 (4), pp. 684-687. Date of Electronic Publication: 2021 Feb 05.
DOI: 10.1111/rda.13896
Abstrakt: In the present work, we established and characterized a 3D functional polarized primary bovine oviduct epithelial cells (BOECs) culture on free-floating type I collagen hydrogels (rafts) at an air-liquid interface (ALI). Intercellular junctions, ultrastructural cellular morphology and the expression of the OVGP1 closely recapitulated those of the in vivo epithelium lining. These morphological and physiological epithelial cell features were maintained under standard DMEM/F12 with 10% foetal bovine serum culture medium for at least 28 days of ALI culture. The versatility of the BOECs raft cultures should allow testing of toxicity compounds, in vitro evaluation of physiological or pathological oviductal states, and the study of epithelial-mesenchymal interactions that are critical for the maintenance of oviductal homeostasis.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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