Lipidomics dataset of PTEN deletion-induced optic nerve regeneration mouse model.

Autor:	Arcuri J; Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, 33136, USA.; Miami Integrative Metabolomics Research Center, Miami, FL, 33136, USA.; Molecular Cellular Pharmacology Graduate Program, University of Miami, Miami, FL, 33136, USA., Hegarty S; F.M. Kirby Neurobiology Center, Department of Neurology, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA., He Z; F.M. Kirby Neurobiology Center, Department of Neurology, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA., Bhattacharya SK; Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, 33136, USA.; Miami Integrative Metabolomics Research Center, Miami, FL, 33136, USA.; Molecular Cellular Pharmacology Graduate Program, University of Miami, Miami, FL, 33136, USA.
Jazyk:	angličtina
Zdroj:	Data in brief [Data Brief] 2020 Dec 26; Vol. 34, pp. 106699. Date of Electronic Publication: 2020 Dec 26 (Print Publication: 2021).
DOI:	10.1016/j.dib.2020.106699
Abstrakt:	The optic nerve is part of the mammalian adult central nervous system (CNS) and has limited capability to regenerate after injury. Deletion of phosphatase and tensin homolog (PTEN), a negative regulator of the PI3 kinase/Akt pathway, has been shown to promote regeneration in retinal ganglion cells (RGCs) after optic nerve injury [1]. We present the lipidome of adult PTEN loxP/loxP mice subjected to intravitreal injection of adeno-associated viruses expressing Cre (AAV-Cre) as a model of CNS neuroregeneration. At 4 weeks old, PTEN loxP/loxP mice were intravitreally-injected with 2-3 μl of either AAV-Cre (KO) or AAV-PLAP (control), and two weeks later optic nerve crush was performed. At indicated time-points after crush (0 days, 7 days, 14 days), mice were euthanized and optic nerves were immediately dissected out, and then flash frozen on dry ice. A modified Bligh and Dyer [2] method was used for lipid extraction from the optic nerves, followed by liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Accela 600 HPLC. The raw scans were analysed with LipidSearch 4.2 and the statistical analysis was conducted through Metaboanalyst 4.0. This data is available at Metabolomics Workbench, study ID ST001477. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article. (© 2020 The Authors.)
Databáze:	MEDLINE
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