Cytotoxicity and Antibacterial Activity of Alginate Hydrogel Containing Nitric Oxide Donor and Silver Nanoparticles for Topical Applications.

Autor: Urzedo AL; Center for Natural and Human Sciences (CCNH), Universidade Federal do ABC (UFABC), CEP 09210-580, Santo André, São Paulo, Brazil., Gonçalves MC; Department of Microbiology, Universidade Estadual de Londrina (UEL), Campus Universitário, CEP 86055-990, Londrina, Paraná, Brazil., Nascimento MHM; Center for Natural and Human Sciences (CCNH), Universidade Federal do ABC (UFABC), CEP 09210-580, Santo André, São Paulo, Brazil., Lombello CB; Center for Engineering, Modeling and Applied Social Sciences, Universidade Federal do ABC (UFABC), CEP 09210-580, Santo André, São Paulo, Brazil., Nakazato G; Department of Microbiology, Universidade Estadual de Londrina (UEL), Campus Universitário, CEP 86055-990, Londrina, Paraná, Brazil., Seabra AB; Center for Natural and Human Sciences (CCNH), Universidade Federal do ABC (UFABC), CEP 09210-580, Santo André, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2020 Apr 13; Vol. 6 (4), pp. 2117-2134. Date of Electronic Publication: 2020 Mar 05.
DOI: 10.1021/acsbiomaterials.9b01685
Abstrakt: Nitric oxide (NO) and silver nanoparticles (AgNPs) are well-known for their antibacterial activity. In this work, S-nitroso-mercaptosuccinic acid (S-nitroso-MSA), a NO donor, and green tea synthesized AgNPs were individually or simultaneously incorporated into alginate hydrogel for topical antibacterial applications. The obtained hydrogels were characterized and the NO release and diffusion of AgNPs and S-nitroso-MSA from alginate hydrogels were investigated. The hydrogels showed a concentration dependent toxicity toward Vero cells. The potent antibacterial effect of the hydrogels was demonstrated toward Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Streptococcus mutans UA159. Interestingly, the combination of S-nitroso-MSA and AgNPs into alginate hydrogels had a superior antibacterial effect, compared with hydrogels containing S-nitroso-MSA or AgNPs individually. This is the first report to describe the synthesis, cytotoxicity, and antibacterial effects of alginate hydrogel containing NO donor and AgNPs. These hydrogels might find important local applications in the combat of bacterial infections.
Databáze: MEDLINE
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