| Autor: |
de Sousa FCB; Department of Animal Science, Universidade Federal do Piauí, Campus Profª Cinobelina Elvas, Bom Jesus, PI, 64900-000, Brazil., do Nascimento CS; Department of Animal Science, Universidade Federal de Sergipe, São Cristóvão, SE, 49100-000, Brazil., Macário MDS; Department of Animal Science, Universidade Federal de Sergipe, São Cristóvão, SE, 49100-000, Brazil., Araújo RDS; Department of Entomology, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil. renandosantosaraujo@gmail.com., Barbosa LT; Department of Animal Science, Universidade Federal de Sergipe, São Cristóvão, SE, 49100-000, Brazil., Bayão GFV; Department of Animal Science, Instituto Federal de Educação, Ciência e Tecnologia do Maranhão, São Luís, MA, 65095-460, Brazil., Sousa KRS; Department of Oceanography and Limnology, Universidade Federal do Maranhão, São Luís, MA, 65080-805, Brazil. |
| Abstrakt: |
Coturniculture has been standing out as an industrial poultry activity in several countries around the world because of the several adaptive advantages of quails. Research that considers the analysis of gene expression can enhance this activity. This study aimed to analyze the stability of reference genes (RGs) in different tissues of quails (both males and females) for the recommendation of use in gene expression studies by the quantitative reverse transcription-polymerase chain reaction (RT-qPCR). The expression stability of ten RGs (ACTA1, ACTB, B2M, GAPDH, HMBS, SDHA, HPRT1, MRPS27, MRPS30, and RPL5) was analyzed in four tissues (breast muscle, abdominal fat, liver, and intestine), and assessed using the statistical tools geNorm, NormFinder, comparative ΔC q method, and BestKeeper. The HPRT1 gene was the most stable in all quail tissues tested, followed by MRPS27 and MRPS30 in breast muscle, B2M and RPL5 in abdominal fat, HMBS and B2M in the liver, and RPL5 and HMBS in the intestine. These results may help studies using RT-qPCR assays to assess quail tissues from both sexes because they provide data on the most stable genes, which should be tested as candidate RGs for other experimental conditions. |
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