TFIIB-related factor 2 regulates glucose-regulated protein 78 expression in acquired middle ear cholesteatoma.

Autor: Sui R; Department of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China; National Health Commission Key Laboratory of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China., Liu C; Hearing reconstruction Key Laboratory of Shandong province/Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 324 Jingwu Weiqi Road, Jinan, Shandong, China; Institute of Medical Sciences, The Second Hospital of Shandong University, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China., Wang N; Department of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China; National Health Commission Key Laboratory of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China., Fan X; Department of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China; National Health Commission Key Laboratory of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China., Han S; Department of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China; National Health Commission Key Laboratory of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China., Zhang J; Department of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China; National Health Commission Key Laboratory of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China., Hou L; Department of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China; National Health Commission Key Laboratory of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China., Zhang X; Department of Otolaryngology, The First People's Hospital of Jining, 6 Health Road, Jining, Shandong, China., Xu A; Department of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China; National Health Commission Key Laboratory of Otolaryngology, The Second hospital, Cheeloo College of Medicine, Shandong University, 274 Beiyuan Road, Jinan, Shandong, China. Electronic address: antingxu@sdu.edu.cn.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Feb 12; Vol. 540, pp. 95-100. Date of Electronic Publication: 2021 Jan 13.
DOI: 10.1016/j.bbrc.2020.12.052
Abstrakt: Acquired middle ear cholesteatoma leads to hearing loss, ear discharge, ear pain, and more serious intracranial complications. However, there is still no effective treatment other than surgery. TFIIB-related factor 2 (BRF2) acted as a redox sensor overexpressing in oxidative stress which linked endoplasmic reticulum (ER) stress, while glucose-regulated protein 78 (GRP78) was a biomarker of ER stress in cancer, atherosclerosis and inflammation. In our study, we investigated the roles of BRF2 and GRP78 in acquired middle ear cholesteatoma. Our results revealed that the expression of BRF2 was significant increased in acquired middle ear cholesteatoma, and which was positively correlated with the expression of GRP78. In addition, BRF2 and GRP78 showed colocalization in epithelium of acquired middle ear cholesteatomas and HaCaT cells. Prolongation of LPS stimulation in HaCaT cells escalated the expression of BRF2 and GRP78. To confirm the role of BRF2 and GRP78, we transfected si-BRF2 into HaCaT cells. All results indicated that BRF2 expression positively regulates the expression of GRP78 and may participate in the pathogenesis of acquire middle ear cholesteatoma.
Competing Interests: Declaration of competing interest We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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