First report of the infectious spleen and kidney necrosis virus (ISKNV) infection in ornamental fishes in India.

Autor: Girisha SK; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India., Kushala KB; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India., Nithin MS; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India., Puneeth TG; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India., Naveen Kumar BT; Department of Aquatic Environment, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India., Vinay TN; Indian Council of Agricultural Research - Central Institute of Brackishwater Aquaculture, Chennai, India., Suresh T; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India., Ajay SK; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India., Venugopal MN; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India., Ramesh KS; College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India.
Jazyk: angličtina
Zdroj: Transboundary and emerging diseases [Transbound Emerg Dis] 2021 Mar; Vol. 68 (2), pp. 964-972. Date of Electronic Publication: 2020 Sep 05.
DOI: 10.1111/tbed.13793
Abstrakt: Infectious spleen and kidney necrosis virus (ISKNV), a member of family iridoviridae, reported for the first time in a wide range of ornamental fish species in India. Significant mortalities during the year 2018-19 were reported from a number of retailers in the region with various clinical signs. The samples of moribund, dead and apparently healthy ornamental fishes were collected from retailers, located in three districts of Karnataka, India. Out of 140 fish samples, 16 samples (11.42%) representing 10 different fish species were found positive to ISKNV by OIE listed primers and same samples were reported to amplify the major capsid protein (MCP) gene of ISKNV. Further, sequence analysis of MCP gene showed that all strains detected in this study were closely related to other documented isolates from different countries with an identity ranging from 98.76% to 100%. Further, they clustered in the clade of ISKNV, during the phylogenetic analysis. The sequence similarity was high (99.94%) to ISKNV strains from Japan, Australia and Malaysia. This is the first report of an ISKNV infection in India. Moreover, out of 10 ISKNV-positive fish species, three species were reported positive to ISKNV for the first time in the world. Further, the in vitro experiment showed the growth of virus in Asian sea bass cell line, which is a natural host of ISKNV. Therefore, considering the lethal nature of megalocytiviruses to infect a vast range of species, proper biosecurity measures need to be taken to control these emerging pathogens.
(© 2020 Wiley-VCH GmbH.)
Databáze: MEDLINE
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