Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats.

Autor:	de Sousa Neto IV; Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil., Durigan JLQ; Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil.; Graduate Program in Rehabilitation Sciences, Universidade de Brasília, Distrito Federal, Brazil., Carreiro de Farias Junior G; Graduate Program in Rehabilitation Sciences, Universidade de Brasília, Distrito Federal, Brazil., Bogni FH; Department of Physiological Sciences, Universidade Federal de São Carlos, São Paulo, Brazil., Ruivo AL; Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil., de Araújo JO; Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil., Nonaka KO; Department of Physiological Sciences, Universidade Federal de São Carlos, São Paulo, Brazil., Selistre-de-Araújo H; Department of Physiological Sciences, Universidade Federal de São Carlos, São Paulo, Brazil., Marqueti RC; Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil.; Graduate Program in Rehabilitation Sciences, Universidade de Brasília, Distrito Federal, Brazil.
Jazyk:	angličtina
Zdroj:	Clinical interventions in aging [Clin Interv Aging] 2021 Jan 08; Vol. 16, pp. 71-81. Date of Electronic Publication: 2021 Jan 08 (Print Publication: 2021).
DOI:	10.2147/CIA.S276518
Abstrakt:	Background: Aging decreases osteogenic ability, inducing harmful effects on the bone extracellular matrix (ECM), while exercise training has been indicated as a tool to counteract bone disorders related to advancing age. The modulation of bone ECM is regulated by several types of matrix metalloproteinase (MMP); however, MMP-2 activity in different trabecular bones in response to resistance training (RT) has been neglected. Remodeling differs in different bones under the application of the same mechanical loading. Thus, we investigated the effects of 12 weeks of RT on MMP-2 activity in the lumbar vertebra (L6), tibia, and femur of young (3 months) and older rats (21 months). Methods: Twenty Wistar rats were divided into four groups (five animals per group): young sedentary or trained and older sedentary or trained. The 12-week RT consisted of climbing a 1.1-m vertical ladder three times per week with progressive weights secured to the animals' tails. The animals were killed 48 h after the end of the experimental period. The MMP-2 activity was assessed by the zymography method. Results: The aging process induced lower MMP-2 activity in the lumbar vertebrae and tibia ( p =0.01). RT upregulated pro, intermediate, and active MMP-2 activity in the tibia of young rats ( p =0.001). RT also upregulated pro and active MMP-2 activity in the lumbar vertebrae and tibia with advancing age ( p =0.01). There was no significant difference ( p >0.05) between groups for MMP-2 of the femur, regardless of age and RT. Conclusion: The aging process impairs MMP-2 activity, but RT is a potential therapeutic approach to minimize the deleterious effects of ECM degeneration in different aged bones. Distinct MMP-2 responses to exercise training may result in specific remodeling processes. Competing Interests: The authors declare that they have no competing interests. (© 2021 de Sousa Neto et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu