| Autor: |
Azamor T; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil.; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., da Silva AMV; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Melgaço JG; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Dos Santos AP; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Xavier-Carvalho C; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Alvarado-Arnez LE; National Research Coordination, Universidad Privada Franz Tamayo, Cochabamba 4780, Bolivia., Batista-Silva LR; Molecular Carcinogenesis Program-Research Coordination, Instituto Nacional do Câncer, Rio de Janeiro 20231-050, Brazil., de Souza Matos DC; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Bayma C; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Missailidis S; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Ano Bom APD; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., Moraes MO; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil., da Costa Neves PC; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil. |
| Abstrakt: |
The yellow fever vaccine (YF17DD) is highly effective with a single injection conferring protection for at least 10 years. The YF17DD induces polyvalent responses, with a TH1/TH2 CD4 + profile, robust T CD8 + responses, and synthesis of interferon-gamma (IFN-γ), culminating in high titers of neutralizing antibodies. Furthermore, C-type lectin domain containing 5A (CLEC5A) has been implicated in innate outcomes in other flaviviral infections. Here, we conducted a follow-up study in volunteers immunized with YF17DD, investigating the humoral response, cellular phenotypes, gene expression, and single nucleotide polymorphisms (SNPs) of IFNG and CLEC5A, to clarify the role of these factors in early response after vaccination. Activation of CLEC5A + monocytes occurred five days after vaccination (DAV). Following, seven DAV data showed activation of CD4 + and CD8 + T cells together with early positive correlations between type II IFN and genes of innate antiviral response (STAT1, STAT2, IRF7, IRF9, OAS1, and RNASEL) as well as antibody levels. Furthermore, individuals with genotypes rs2430561 AT/AA, rs2069718 AG/AA (IFNG), and rs13237944 AC/AA (CLEC5A), exhibited higher expression of IFNG and CLEC5A, respectively. Together, we demonstrated that early IFN-γ and CLEC5A responses, associated with rs2430561, rs2069718, and rs13237944 genotypes, may be key mechanisms in the long-lasting immunity elicited by YF17DD. |
