Phospholipase A2 enzyme from the venom of Egyptian honey bee Apis mellifera lamarckii with anti-platelet aggregation and anti-coagulation activities.

Autor: Darwish DA; Molecular Biology Department, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt., Masoud HMM; Molecular Biology Department, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt. hssnmasoud@yahoo.com., Abdel-Monsef MM; Molecular Biology Department, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt., Helmy MS; Molecular Biology Department, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt., Zidan HA; Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt., Ibrahim MA; Molecular Biology Department, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt.
Jazyk: angličtina
Zdroj: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2021 Jan 14; Vol. 19 (1), pp. 10. Date of Electronic Publication: 2021 Jan 14.
DOI: 10.1186/s43141-020-00112-z
Abstrakt: Background: Honey bee venom contains various enzymes with wide medical and pharmaceutical applications.
Results: The phospholipase A2 (PLA2) has been apparently purified from the venom of Egyptian honey bee (Apis mellifera lamarckii) 8.9-fold to a very high specific activity of 6033 U/mg protein using DEAE-cellulose and Sephacryl S-300 columns. The purified bee venom PLA2 is monomeric 16 kDa protein and has isoelectric point (pI) of 5.9. The optimal activity of bee venom PLA2 was attained at pH 8 and 45 °C. Cu 2+ , Ni 2+ , Fe 2+ , Ca 2+ , and Co 2+ exhibited a complete activating effect on it, while Zn 2+ , Mn 2+ , NaN 3 , PMSF, N-Methylmaleimide, and EDTA have inhibitory effect.
Conclusions: The purified bee venom PLA2 exhibited anti-platelet aggregation and anti-coagulation activities which makes it promising agent for developing novel anti-clot formation drugs in future.
Databáze: MEDLINE
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