A Novel N4-Like Bacteriophage Isolated from a Wastewater Source in South India with Activity against Several Multidrug-Resistant Clinical Pseudomonas aeruginosa Isolates.

Autor:	Menon ND; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., Kumar MS; Amrita Biosensor Research Lab, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu, India.; Department of Sciences, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu, India., Satheesh Babu TG; Amrita Biosensor Research Lab, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu, India.; Department of Sciences, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu, India., Bose S; Icon Analytical Private Limited, Worli, Mumbai, Maharashtra, India.; Institute for Stem Cell Science and Regenerative Medicine, Bangalore, Karnataka, India., Vijayakumar G; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., Baswe M; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., Chatterjee M; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., D'Silva JR; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., Shetty K; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., Haripriyan J; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India., Kumar A; Department of Microbiology, Amrita Institute of Medical Sciences, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India., Nair S; Department of Microbiology, DDRC SRL Diagnostic Private Limited, Trivandrum, Kerala, India., Somanath P; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., Nair BG; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India., Nizet V; Department of Pharmacology, University of California, San Diego, La Jolla, California, USA.; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, USA.; Department of Pediatrics, University of California, San Diego, La Jolla, California, USA., Kumar GB; School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, India gkumar@am.amrita.edu.; Tata Institute for Genetics and Society-India (TIGS), TIGS Center at inStem, Bangalore, Karnataka, India.
Jazyk:	angličtina
Zdroj:	MSphere [mSphere] 2021 Jan 13; Vol. 6 (1). Date of Electronic Publication: 2021 Jan 13.
DOI:	10.1128/mSphere.01215-20
Abstrakt:	Multidrug-resistant community-acquired infections caused by the opportunistic human pathogen Pseudomonas aeruginosa are increasingly reported in India and other locations globally. Since this organism is ubiquitous in the environment, samples such as sewage and wastewater are rich reservoirs of P. aeruginosa bacteriophages. In this study, we report the isolation and characterization of a novel P. aeruginosa N4-like lytic bacteriophage, vB_Pae_AM.P2 (AM.P2), from wastewater in Kerala, India. AM.P2 is a double-stranded DNA podovirus that efficiently lyses the model strain, PAO1, at a multiplicity of infection as low as 0.1 phage per bacterium and resistance frequency of 6.59 × 10 -4 Synergy in bactericidal activity was observed between AM.P2 and subinhibitory concentrations of the antibiotic ciprofloxacin. Genome sequencing of AM.P2 revealed features similar to those of the N4-like P. aeruginosa phages LUZ7 and KPP21. As judged by two independent assay methods, spot tests and growth inhibition, AM.P2 successfully inhibited the growth of almost 30% of strains from a contemporary collection of multidrug-resistant P. aeruginosa clinical isolates from South India. Thus, AM.P2 may represent an intriguing candidate for inclusion in bacteriophage cocktails developed for various applications, including water decontamination and clinical bacteriophage therapy. IMPORTANCE In India, multidrug resistance determinants are much more abundant in community-associated bacterial pathogens due to the improper treatment of domestic and industrial effluents. In particular, a high bacterial load of the opportunistic pathogen P. aeruginosa in sewage and water bodies in India is well documented. The isolation and characterization of bacteriophages that could target emerging P. aeruginosa strains, representing possible epicenters for community-acquired infections, could serve as a useful alternative tool for various applications, such as phage therapy and environmental treatment. Continuing to supplement the repertoire of broad-spectrum bacteriophages is an essential tool in confronting this problem. (Copyright © 2021 Menon et al.)
Databáze:	MEDLINE
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