Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor.

Autor: Panchenko PA; Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia.; Department of Technology of Fine Organic Synthesis and Chemistry of Dyes, Dmitry Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia., Efremenko AV; Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia.; Laboratory of Optical Microscopy and Spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, 117997 Moscow, Russia., Feofanov AV; Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia.; Laboratory of Optical Microscopy and Spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, 117997 Moscow, Russia., Ustimova MA; Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia., Fedorov YV; Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia., Fedorova OA; Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia.; Department of Technology of Fine Organic Synthesis and Chemistry of Dyes, Dmitry Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia.
Jazyk: angličtina
Zdroj: Sensors (Basel, Switzerland) [Sensors (Basel)] 2021 Jan 11; Vol. 21 (2). Date of Electronic Publication: 2021 Jan 11.
DOI: 10.3390/s21020470
Abstrakt: Bis(styryl) dye 1 bearing N -phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe 1 selectively responds to the presence of Hg 2+ via the changes in the emission intensity as well as in the emission band shape, which is a result of formation of the complex with 1:1 metal to ligand ratio (dissociation constant 0.56 ± 0.15 µM). The sensing mechanism is based on the interplay between the RET (resonance energy transfer) and ICT (intramolecular charge transfer) interactions occurring upon the UV/Vis (380 or 405 nm) photoexcitation of both styryl chromophores in probe 1. Bio-imaging studies revealed that the yellow (500-600 nm) to red (600-730 nm) fluorescence intensity ratio decreased from 4.4 ± 0.2 to 1.43 ± 0.10 when cells were exposed to increasing concentration of mercury (II) ions enabling ratiometric quantification of intracellular Hg 2+ concentration in the 37 nM-1 μM range.
Databáze: MEDLINE
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