Meiotic synapsis of homeologous chromosomes and mismatch repair protein detection in the parthenogenetic rock lizard Darevskia unisexualis.

Autor: Spangenberg V; Vavilov Institute of General Genetics RAS, Moscow, Russia., Arakelyan M; Department of Zoology, Yerevan State University, Yerevan, Armenia., Galoyan E; Severtsov Institute of Ecology and Evolution RAS, Moscow, Russia., Martirosyan I; Institute of Gene Biology, RAS, Moscow, Russia., Bogomazova A; Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Moscow, Russia., Martynova E; Skolkovo Institute of Science and Technology, Moscow, Russia., de Bello Cioffi M; Laboratório de Citogenética de Peixes, UniversidadeFederal de São Carlos, São Carlos, SP, Brazil., Liehr T; Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany., Al-Rikabi A; Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany., Osipov F; Severtsov Institute of Ecology and Evolution RAS, Moscow, Russia., Petrosyan V; Severtsov Institute of Ecology and Evolution RAS, Moscow, Russia., Kolomiets O; Vavilov Institute of General Genetics RAS, Moscow, Russia.
Jazyk: angličtina
Zdroj: Molecular reproduction and development [Mol Reprod Dev] 2021 Feb; Vol. 88 (2), pp. 119-127. Date of Electronic Publication: 2021 Jan 12.
DOI: 10.1002/mrd.23450
Abstrakt: Parthenogenetic species of Caucasian rock lizards of the genus Darevksia are important evidence for reticulate evolution and speciation by hybridization in vertebrates. Female-only lineages formed through interspecific hybridization have been discovered in many groups. Nevertheless, critical mechanisms of oogenesis and specifics of meiosis that provide long-term stability of parthenogenetic species are still unknown. Here we report cytogenetic characteristics of somatic karyotypes and meiotic prophase I nuclei in the diploid parthenogenetic species Darevskia unisexualis from the new population "Keti" in Armenia which contains an odd number of chromosomes 2n = 37, instead of the usual 2n = 38. We revealed 36 acrocentric chromosomes and a single metacentric autosomal chromosome, resulting from Robertsonian translocation. Comparative genomic hybridization revealed that chromosome fusion occurred between two chromosomes inherited from the maternal species, similar to another parthenogenetic species D. rostombekowi. To trace the chromosome behaviour in meiosis, we performed an immunocytochemical study of primary oocytes' spread nuclei and studied chromosome synapsis during meiotic prophase I in D. unisexualis based on analysis of synaptonemal complexes (SCs). We found meiotic SC-trivalent composed of one metacentric and two acrocentric chromosomes. We confirmed that the SC was assembled between homeologous chromosomes inherited from two parental species. Immunostaining of the pachytene and diplotene nuclei revealed a mismatch repair protein MLH1 loaded to all autosomal SC bivalents. Possible mechanisms of meiotic recombination between homeologous chromosomes are discussed.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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