Korean Red Ginseng aqueous extract improves markers of mucociliary clearance by stimulating chloride secretion.

Autor: Cho DY; Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States.; Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama, United States., Skinner D; Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States., Zhang S; Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States., Lazrak A; Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama, United States.; Department of Anesthesiology and Perioperative Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States., Lim DJ; Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States., Weeks CG; Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States., Banks CG; Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States., Han CK; Korea Ginseng Research Institute, Korea Ginseng Corporation, Daejeon, Republic of Korea., Kim SK; Department of Biomedical Chemistry, Konkuk University, Chungju, Republic of Korea., Tearney GJ; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States., Matalon S; Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama, United States.; Department of Anesthesiology and Perioperative Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States., Rowe SM; Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama, United States.; Departments of Medicine, Pediatrics, Cell Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, Alabama, United States., Woodworth BA; Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, United States.; Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama, United States.
Jazyk: angličtina
Zdroj: Journal of ginseng research [J Ginseng Res] 2021 Jan; Vol. 45 (1), pp. 66-74. Date of Electronic Publication: 2019 Sep 13.
DOI: 10.1016/j.jgr.2019.09.001
Abstrakt: Background: Abnormal chloride (Cl - ) transport has a detrimental impact on mucociliary clearance in both cystic fibrosis (CF) and non-CF chronic rhinosinusitis. Ginseng is a medicinal plant noted to have anti-inflammatory and antimicrobial properties. The present study aims to assess the capability of red ginseng aqueous extract (RGAE) to promote transepithelial Cl - secretion in nasal epithelium.
Methods: Primary murine nasal septal epithelial (MNSE) [wild-type (WT) and transgenic CFTR -/- ], fisher-rat-thyroid (FRT) cells expressing human WT CFTR, and TMEM16A-expressing human embryonic kidney cultures were utilized for the present experiments. Ciliary beat frequency (CBF) and airway surface liquid (ASL) depth measurements were performed using micro-optical coherence tomography (μOCT). Mechanisms underlying transepithelial Cl - transport were determined using pharmacologic manipulation in Ussing chambers and whole-cell patch clamp analysis.
Results: RGAE (at 30μg/mL of ginsenosides) significantly increased Cl - transport [measured as change in short-circuit current (ΔI SC  = μA/cm 2 )] when compared with control in WT and CFTR -/- MNSE (WT vs control = 49.8±2.6 vs 0.1+/-0.2, CFTR -/-  = 33.5±1.5 vs 0.2±0.3, p < 0.0001). In FRT cells, the CFTR-mediated ΔI SC attributed to RGAE was small (6.8 ± 2.5 vs control, 0.03 ± 0.01, p < 0.05). In patch clamp, TMEM16A-mediated currents were markedly improved with co-administration of RGAE and uridine 5-triphosphate (8406.3 +/- 807.7 pA) over uridine 5-triphosphate (3524.1 +/- 292.4 pA) or RGAE alone (465.2 +/- 90.7 pA) (p < 0.0001). ASL and CBF were significantly greater with RGAE (6.2+/-0.3 μ m vs control, 3.9+/-0.09 μ m; 10.4+/-0.3 Hz vs control, 7.3 ± 0.2 Hz; p  < 0.0001) in MNSE.
Conclusion: RGAE augments ASL depth and CBF by stimulating Cl - secretion through CaCC, which suggests therapeutic potential in both CF and non-CF chronic rhinosinusitis.
(© 2019 The Korean Society of Ginseng, Published by Elsevier Korea LLC.)
Databáze: MEDLINE
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