Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training.

Autor: Deshmukh AS; The Novo Nordisk Foundation Center for Protein Research, Clinical Proteomics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. atul.deshmukh@sund.ku.dk.; The Novo Nordisk Foundation Center for Basic Metablic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. atul.deshmukh@sund.ku.dk., Steenberg DE; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark., Hostrup M; Section of Integrative Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark., Birk JB; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark., Larsen JK; The Novo Nordisk Foundation Center for Basic Metablic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Santos A; The Novo Nordisk Foundation Center for Protein Research, Clinical Proteomics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Kjøbsted R; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark., Hingst JR; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark., Schéele CC; The Novo Nordisk Foundation Center for Basic Metablic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.; The Centre of Inflammation and Metabolism and Centre for Physical Activity Research Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark., Murgia M; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.; Department of Biomedical Sciences, University of Padua, Padua, Italy., Kiens B; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark., Richter EA; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark., Mann M; The Novo Nordisk Foundation Center for Protein Research, Clinical Proteomics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany., Wojtaszewski JFP; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark. jwojtaszewski@nexs.ku.dk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Jan 12; Vol. 12 (1), pp. 304. Date of Electronic Publication: 2021 Jan 12.
DOI: 10.1038/s41467-020-20556-8
Abstrakt: Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca 2+ signaling, and fat and glucose metabolism adapts to training in a fiber type-specific manner. Our data provide a resource to elucidate molecular mechanisms underlying muscle function and health, and our workflow allows fiber type-specific proteomic analyses of snap-frozen non-embedded human muscle biopsies.
Databáze: MEDLINE
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