Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing.

Autor:	Liang D; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Xue J; Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China., Geng L; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Zhou L; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Lv B; Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China., Zeng Q; Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China., Xiong K; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Zhou H; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Xie D; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Zhang F; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Liu J; Translational Center of Stem Cell Research, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China., Liu Y; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China., Li L; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China.; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai, 200092, China., Yang J; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China.; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai, 200092, China., Xue Z; Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China. xuezg@tongji.edu.cn.; Reproductive Medicine Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China. xuezg@tongji.edu.cn., Chen YH; Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China. chenyh@cashq.ac.cn.; Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China. chenyh@cashq.ac.cn.; Institute of Medical Genetics, Tongji University, Shanghai, 200092, China. chenyh@cashq.ac.cn.; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai, 200092, China. chenyh@cashq.ac.cn.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2021 Jan 12; Vol. 12 (1), pp. 287. Date of Electronic Publication: 2021 Jan 12.
DOI:	10.1038/s41467-020-20448-x
Abstrakt:	Bioelectrical impulses intrinsically generated within the sinoatrial node (SAN) trigger the contraction of the heart in mammals. Though discovered over a century ago, the molecular and cellular features of the SAN that underpin its critical function in the heart are uncharted territory. Here, we identify four distinct transcriptional clusters by single-cell RNA sequencing in the mouse SAN. Functional analysis of differentially expressed genes identifies a core cell cluster enriched in the electrogenic genes. The similar cellular features are also observed in the SAN from both rabbit and cynomolgus monkey. Notably, Vsnl1, a core cell cluster marker in mouse, is abundantly expressed in SAN, but is barely detectable in atrium or ventricle, suggesting that Vsnl1 is a potential SAN marker. Importantly, deficiency of Vsnl1 not only reduces the beating rate of human induced pluripotent stem cell - derived cardiomyocytes (hiPSC-CMs) but also the heart rate of mice. Furthermore, weighted gene co-expression network analysis (WGCNA) unveiled the core gene regulation network governing the function of the SAN in mice. Overall, these findings reveal the whole transcriptome profiling of the SAN at single-cell resolution, representing an advance toward understanding of both the biology and the pathology of SAN.
Databáze:	MEDLINE
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