Effect of oxytocin on lipid accumulation under inflammatory conditions in human macrophages.

Autor: Karten A; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Vernice NA; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Renna HA; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Carsons SE; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., DeLeon J; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Pinkhasov A; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Gomolin IH; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Glass DS; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Reiss AB; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America., Kasselman LJ; NYU Langone Hospital - Long Island Biomedical Research Institute and NYU Long Island School of Medicine, Mineola, New York, United States of America. Electronic address: lora.kasselman@nyulangone.org.
Jazyk: angličtina
Zdroj: Experimental and molecular pathology [Exp Mol Pathol] 2021 Feb; Vol. 118, pp. 104604. Date of Electronic Publication: 2021 Jan 09.
DOI: 10.1016/j.yexmp.2021.104604
Abstrakt: Introduction and Aims: Oxytocin (OT) is a neuropeptide hormone secreted by the posterior pituitary gland. Deficits in OT action have been observed in patients with behavioral and mood disorders, some of which correlate with an increased risk of cardiovascular disease (CVD). Recent research has revealed a wider systemic role that OT plays in inflammatory modulation and development of atherosclerotic plaques. This study investigated the role that OT plays in cholesterol transport and foam cell formation in LPS-stimulated THP-1 human macrophages.
Methods: THP-1 differentiated macrophages were treated with media, LPS (100 ng/ml), LPS + OT (10 pM), or LPS + OT (100 pM). Changes in gene expression and protein levels of cholesterol transporters were analyzed by real time quantitative PCR (RT-qPCR) and Western blot, while oxLDL uptake and cholesterol efflux capacity were evaluated with fluorometric assays.
Results: RT-qPCR analysis revealed a significant increase in ABCG1 gene expression upon OT + LPS treatment, compared to LPS alone (p = 0.0081), with Western blotting supporting the increase in expression of the ABCG1 protein. Analysis of oxLDL uptake showed a significantly lower fluorescent value in LPS + OT (100pM) -treated cells when compared to LPS alone (p < 0.0001). While not statistically significant (p = 0.06), cholesterol efflux capacity increased with LPS + OT treatment.
Conclusion: We demonstrate here that OT can attenuate LPS-mediated lipid accumulation in THP-1 macrophages. These findings support the hypothesis that OT could be used to reduce pro-inflammatory and potentially atherogenic changes observed in patients with heightened CVD risk. This study suggests further exploration of OT effects on monocyte and macrophage cholesterol handling in vivo.
(Copyright © 2021. Published by Elsevier Inc.)
Databáze: MEDLINE
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