| Autor: |
García-Montelongo M; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Apartado postal 629, 36500, Irapuato, Guanajuato, Mexico., González-Villarreal SE; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Apartado postal 629, 36500, Irapuato, Guanajuato, Mexico., Del Rincón-Castro MC; División de Ciencias de la Vida, Departamento de Alimentos, Universidad de Guanajuato, 36500, Irapuato, Guanajuato, Mexico., Ibarra JE; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Apartado postal 629, 36500, Irapuato, Guanajuato, Mexico. jorge.ibarra@cinvestav.mx. |
| Abstrakt: |
Bacillus thuringiensis is a potential control agent for plant-parasitic nematodes. Nematode intestinal receptors for Cry21-type toxins are poorly known. Therefore, a strategy was tested as a primary screening tool to find possible Cry toxin receptors, using a nematicidal Bt strain and the RNAi technique on Caenorhabditis elegans. Six genes encoding intestinal membrane proteins were selected (abt-4, bre-1, bre-2, bre-3, asps-1, abl-1) as possible targets for Cry proteins. Fractions of each selected gene were amplified by PCR. Amplicons were cloned into the L4440 vector to transform the E. coli HT155 (DE3) strain. Transformed bacteria were used to silence the selected genes using the RNAi feeding method. Nematodes with silenced genes were tested with the Bt strain LBIT-107, which harbors the nematicidal protein Cry21Aa3, among others. Results indicated that nematodes with the silenced abt-4 gene were 69.5% more resistant to the LBIT-107 strain, in general, and 79% to the Cry21Aa3 toxin, specifically. |
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