Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice.

Autor: Lau SKP; Department of Microbiology, The University of Hong Kong, Hong Kong, China. skplau@hku.hk.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China. skplau@hku.hk.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China. skplau@hku.hk., Fan RYY; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Zhu L; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Li KSM; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Wong ACP; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Luk HKH; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Wong EYM; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Lam CSF; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Lo GCS; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Fung J; Department of Microbiology, The University of Hong Kong, Hong Kong, China., He Z; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Fok FCH; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Au-Yeung RKH; Department of Pathology, The University of Hong Kong, Hong Kong, China., Zhang L; Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China., Kok KH; Department of Microbiology, The University of Hong Kong, Hong Kong, China., Yuen KY; Department of Microbiology, The University of Hong Kong, Hong Kong, China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China., Woo PCY; Department of Microbiology, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Jan 11; Vol. 12 (1), pp. 216. Date of Electronic Publication: 2021 Jan 11.
DOI: 10.1038/s41467-020-20458-9
Abstrakt: While a number of human coronaviruses are believed to be originated from ancestral viruses in bats, it remains unclear if bat coronaviruses are ready to cause direct bat-to-human transmission. Here, we report the isolation of a MERS-related coronavirus, Tylonycteris-bat-CoV-HKU4, from lesser bamboo bats. Tylonycteris-bat-CoV-HKU4 replicates efficiently in human colorectal adenocarcinoma and hepatocarcinoma cells with cytopathic effects, and can utilize human-dipeptidyl-peptidase-4 and dromedary camel-dipeptidyl-peptidase-4 as the receptors for cell entry. Flow cytometry, co-immunoprecipitation and surface plasmon resonance assays show that Tylonycteris-bat-CoV-HKU4-receptor-binding-domain can bind human-dipeptidyl-peptidase-4, dromedary camel-dipeptidyl-peptidase-4, and Tylonycteris pachypus-dipeptidyl-peptidase-4. Tylonycteris-bat-CoV-HKU4 can infect human-dipeptidyl-peptidase-4-transgenic mice by intranasal inoculation with self-limiting disease. Positive virus and inflammatory changes were detected in lungs and brains of infected mice, associated with suppression of antiviral cytokines and activation of proinflammatory cytokines and chemokines. The results suggest that MERS-related bat coronaviruses may overcome species barrier by utilizing dipeptidyl-peptidase-4 and potentially emerge in humans by direct bat-to-human transmission.
Databáze: MEDLINE
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